Can anyone suggest a good positive control for Complement Dependent Cytotoxicity assay?

05 December 2024 0 9K Report

I am looking for a good commercially available positive control for CDC assay or is there a way to achieve 100% complement mediated killing of peripheral blood mononuclear cells or myeloma cell lines using any Anti CD38. I would be analyzing the death using CFSE for staining target cells with PI/7AAD using flow cytometry. If anyone has any experience with such assays, suggestions are most welcome.

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Science method

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