Gas chromatography would be the preferred method of analysis for that group of samples, not HPLC. Can you use HPLC for this group? Maybe. A C18 column with a mixture of ACN and acidified water plus a RID (isocratic) may resolve all of them on one run. Give it a try.
Thank you very much Of course, the GC method is better, but I do not have a GC device Therefore, it is possible to use an HPLC device with an IR-detector and column-sugar
I ran thymol on C18 using ACN/water. It had a very skewed peak with a long tail. It was necessary to raise the pH to the area of 5 - 6 to get a decent peak shape. If the pH got too high, the skew reversed and showed in front of the peak. I think I changed to a dilute phosphate buffer, but it was 40 years ago, and I don't remember the specifics.
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