For cDNA synthesis from microRNA using a stem-loop primer, is preliminary process necessary before the main synthesis process,like heating the primer?

05 January 2024 0 3K Report

Well, as you know, the stem-loop primer has a long chain, increasing the likelihood of undesirable secondary structure formation.

Badges
Science method

More Reza Ghaffaripour's questions See All

If the emission of all greenhouse gases is stopped now, will the temperature of the earth continue to rise?

Here's a serious question: If we were to stop emitting all greenhouse gases right now, would the Earth's temperature start to cool down, or would the existing greenhouse gases continue to warm the...

30 July 2024 5,121 4 View

How to prepare 100ml solution of 2.5% Glutraldehyde in 0.1M PBS(phosphate buffer) pH about 7, from 25% Glutradehyde and 1X PBS?

final solution volume is 100ml

03 June 2024 417 0 View

How can i use hexamethyldisilazane(HMDS) for drying instead of Co2 and freez drying for SEM analysis of rice anther and pollen?

could you please help with a protocol? should i do it directly on the aluminium stub after dehydration with 100% alcohol or not? should i release the pollen or whole anther is better and dehisce...

03 June 2024 4,793 8 View

What is the best method to enhance the surface hardness of cutting tools made from SPK steel?

SPK tool steels are used for cutting wires made from low carbon steels. We are searching for a cheap and effective method for increasing the cutting characteristics of the cutters.

14 May 2024 4,948 0 View

Constant Reciprocal of Step Size?

Hello I recently encountered a problem with the reciprocal of step size plot in Comsol Multiphysics. After a certain time (seconds) in a time-dependent solver, the plot oscillations will be...

04 May 2024 7,278 0 View

Poor viability of T cell after transduction with CAR Lentivirus!?

Hi everyone I produced a batch of virus , enriched it with centrifugation, and titrated it with T cells after 48h. For my experiments I want to use MOI 3 and that's equal to 1/12 dilution. but at...

02 May 2024 9,727 3 View

Are you interested in submitting papers to a new journal called "InfoScience Trends"?

InfoScience Trends offers comprehensive scientific content that delves into various facets of information science research. This includes but is not limited to topics such as information...

26 April 2024 9,520 1 View

Does the color of pan traps affect the sampling of flies from the superfamily Bombylioidea?

Garret, 2020 used traps of blue, yellow, and white colors for sampling and collected several specimens.

06 April 2024 6,995 2 View

Laboratory procedures for identifying the concentrations of formic acid and acetic acid in aqueous solutions???

What are the laboratory procedures for identifying the concentrations of formic acid and acetic acid in aqueous solutions using titration, spectrophotometry, or gas chromatography methods...

06 April 2024 187 3 View

Is it possible to produce a very small amount of volatile oil along with condensed gas? If so, could you please provide a real example?

Is it possible to produce a very small amount of volatile oil along with condensed gas? If so, could you please provide a real example? Thank you.

04 April 2024 1,411 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,667 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,113 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,056 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,672 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,404 6 View

What should be the sample container for the hydrothermal reaction in a microwave reactor at 180 °C for 10 min at the heating rate of 5 °C per minute?

Suggest one of them 1. Teflon-lined stainless steel autoclave: 2. Alumina (Al2O3) ceramic container

30 July 2024 7,326 1 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 840 2 View

Aqua regia not heating up?

I used Aqua regia to clean Au nanoparticles in the used glassware. But for some reasons, it is not heating up upon mixing. The color did change from no color to light orange. The Au NPs on the...

25 July 2024 4,388 0 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,329 5 View