A forum to address questions regarding methods of protein purification. | Contact experts in Protein Purification to get answers
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Questions related to Protein Purification
I installed CCP4 on my PC (Windows 10), but I can't see graphs in Aimless, phaser and also, Refmac5 is not able to open Coot directly itself. Does anyone experience the same issue? Any...
25 October 2022 7,959 0 View
We are working with a protein with a MW of 12 kDa with a C-terminal "aldehyde-tag" (formyl-glycine) produced as described in: https://pubmed.ncbi.nlm.nih.gov/17450134/ After purification and...
17 October 2022 9,637 0 View
I nucleofected iPSCs from one patient line, grew colonies and attempted to genotype them. i Had previously run a PCR gradient to determine the best Tm for the primers. most of the PCR products...
06 October 2022 5,777 0 View
Hi eveyone, This image is feline adipose-derived mesenchymal stem cell suspension. It becomes like this two hours after preparing the product (It is stored at +4 degrees). Viability of stem cells...
15 September 2022 1,777 1 View
I have isolated cells from murine adipose tissue from 4 month old mice and am growing them in mesencult. I know that MSCs include many cell types but I would like to focus on adipose derived stem...
02 August 2022 7,745 0 View
Hiii Researchers, I have recently started working on exosomes and curious to explore more in this field. Unlike cell lysates where we use housekeeping gene expressions as normalization control,...
10 June 2022 500 2 View
I am looking into increasing the RNA yield of rat brain isolation and was wondering if using lipase would be beneficial and what kind of concentrations are recommended.
07 May 2022 501 0 View
Currently, I am working with two recombinant versions of the protein A (SpA)- one with cysteine and the other without cysteine. On coomassie staining in both cases, the band intensities are...
10 April 2022 8,014 3 View
Hello, I'm looking at HtrA protease activity and don't have any Brij 35 in stock. Is there something else I can use instead of this detergent? Thanks for the response.
07 December 2021 9,620 4 View
Hi there, I have synthesized selenium nanoparticles coated with different concentrations of chitosan folate polymer, but based on dls and fesem, the size of nanoparticles coated with chitosan...
04 October 2021 4,810 1 View
Hello, I'm planning on performing experiments to investigate the lifespan of a membrane used for the concentration and diafiltration of a protein product. In the literature, there is a...
08 September 2021 9,716 0 View
I am exploring potential chemical based approaches for cell cycle synchronization and would like to hear from anyone with experience on this technique what they believe to be the most applicable...
26 April 2021 2,687 3 View
Hi guys, I wondered anyone know how to disassemble ATP synthase complex in vitro by any drug or other method? Thanks,
14 April 2021 9,268 4 View
I measured the OD600 for C. crescentus wild type and mutants strains every 60 minutes, and I need to calculate the doubling time for each strain to see the effect of mutations on doubling time....
26 February 2021 2,238 2 View
I am working on a project where I have to purify some proteins that are tagged with a 6x His tag. Since my proteins were produced by bacteria, I purchased the HOOKTM 6X His Protein Purification...
10 February 2021 7,098 4 View
UV-Visible Spectrophotometric evaluation of crude phycobiliproteins shows absorption peaks corresponding to both phycocyanin and phycoerythrin. so, how can I separate both phycocyanin and...
05 February 2021 8,236 2 View
Hello, I currently have sliced and punched mouse brain tissue using the cryostat and I wish to analyze it for the concentrations of different dopamine receptors. We initially desired to use...
02 January 2021 9,589 2 View
Hi everybody, Does anyone have a good protocol for protein purification using AKTA? I have tried to purify two proteins but after several attempts it was not successful. So, I really appreciate...
29 December 2020 5,438 15 View
Hi everyone, I am doing protein expression via E.coli. I am required to lyse the e.coli cells via sonication to obtain cell lysate. I normally sonicate 1L cell pellet suspended in salt buffer at...
23 December 2020 9,830 7 View
Hi, I am currently working with BL21(DE3)PLySs and PRSET A vector. Out lab frequently transformation and culture E Coli for protein purification. But recently, even though there is colony after...
14 December 2020 9,325 9 View
I have flash-frozen brains and am looking to extract RNA for RNA-seq, and protein for western blotting. I've seen other questions related to this...
11 December 2020 6,438 0 View
Based on this paper The P1 ' specificity of tobacco etch virus protease ,TEV protease can cleave between the Gln and several amino-acids (besides Gly/Ser) with acceptable efficiency in its...
27 November 2020 6,132 4 View
During protein purification, a protease inhibitor is added to the lysate (10ul/mL of lysis buffer). Why is this amount of inhibitor desirable? What is the potential danger to the protein if a...
24 November 2020 8,351 7 View
pure protein , methods to puried , characterized pure protein
17 November 2020 7,459 3 View