A forum to address questions regarding methods of protein purification. | Contact experts in Protein Purification to get answers
1,969 views 1,849 posts
Questions related to Protein Purification
Does any have a method for analyzing VOCs in water? or VOCs solvent? I study VOCs degradation in water by microorganisms.
02 January 2023 8,443 2 View
I am having some trouble with the transformation protocol of electrocompetent TOP10 E. coli cells. At first I thought it could be because of the size of the plasmid (10 kb) so I electroporated...
26 December 2022 7,029 5 View
Hello everyone, I only ask for help on ResearchGate when I can't find a proper answer out there. I want to start experimenting with statins in PBMC cultures and I've been looking for a...
14 December 2022 2,717 2 View
I'm using Ni-Nta beads to purify the His-tag recombinant protein. After sonication, I haven't found any dimers upon SDS-PAGE. But after that load and the next purified eluted protein formed...
06 December 2022 9,311 4 View
am trying to purify a human DNA binding protein (normally localized in nucleus and mitochondria). I am currently optimizing the conditions I tried 2 different approaches: 1. HisTrap-> TEV -->...
06 December 2022 5,111 5 View
Hi, I need to store some larvae of G. mellonella for future RNA extractions. My idea is to pre freeze them with liquid nitrogen and then put them in a eppendorf at -80. Should I add also...
01 December 2022 1,257 3 View
Hi, colleagues. Can someone assist me on how to validate my docking protocol using EF and ROC curves? I have searched through the literature i am failing to find a step-by-step approach to...
29 November 2022 2,517 2 View
I have a couple of proteins (95-130 KDa) to make them clean. What are the best microcentrifuge tube based column filtrations you guys have tried and worked best. Any suggestion with direct product...
29 November 2022 4,428 3 View
Hi all! Is anyone aware of a model protein that crystallizes, albeit slowly? (~3-6 weeks)
25 October 2022 6,951 4 View
I installed CCP4 on my PC (Windows 10), but I can't see graphs in Aimless, phaser and also, Refmac5 is not able to open Coot directly itself. Does anyone experience the same issue? Any...
25 October 2022 8,027 0 View
We are working with a protein with a MW of 12 kDa with a C-terminal "aldehyde-tag" (formyl-glycine) produced as described in: https://pubmed.ncbi.nlm.nih.gov/17450134/ After purification and...
17 October 2022 9,703 0 View
I nucleofected iPSCs from one patient line, grew colonies and attempted to genotype them. i Had previously run a PCR gradient to determine the best Tm for the primers. most of the PCR products...
06 October 2022 5,850 0 View
Hi eveyone, This image is feline adipose-derived mesenchymal stem cell suspension. It becomes like this two hours after preparing the product (It is stored at +4 degrees). Viability of stem cells...
15 September 2022 1,835 1 View
I have isolated cells from murine adipose tissue from 4 month old mice and am growing them in mesencult. I know that MSCs include many cell types but I would like to focus on adipose derived stem...
02 August 2022 7,789 0 View
Hiii Researchers, I have recently started working on exosomes and curious to explore more in this field. Unlike cell lysates where we use housekeeping gene expressions as normalization control,...
10 June 2022 546 2 View
I am looking into increasing the RNA yield of rat brain isolation and was wondering if using lipase would be beneficial and what kind of concentrations are recommended.
07 May 2022 530 0 View
Currently, I am working with two recombinant versions of the protein A (SpA)- one with cysteine and the other without cysteine. On coomassie staining in both cases, the band intensities are...
10 April 2022 8,063 3 View
Where can i Buy Saccharomyces cerevisiae SC strains in pakistan for ethanol production ?
21 March 2022 3,095 3 View
Hello, I'm looking at HtrA protease activity and don't have any Brij 35 in stock. Is there something else I can use instead of this detergent? Thanks for the response.
07 December 2021 9,685 4 View
Pasteur reported that Yeast under anaerobic conditions produces alcohol. Lactic acid contamination suspends fermentation and promotes growth. (see my article Pasteur's dictum in . 2014 Mar...
22 November 2021 311 0 View
Hello everyone I want to know is there any online webserver which can be utilized for blind docking for ssDNA-ligand docking?. I have tried few protein-protein or protein-DNA compatible webservers...
08 November 2021 1,011 5 View
Hi there, I have synthesized selenium nanoparticles coated with different concentrations of chitosan folate polymer, but based on dls and fesem, the size of nanoparticles coated with chitosan...
04 October 2021 4,861 1 View
Hello, I'm planning on performing experiments to investigate the lifespan of a membrane used for the concentration and diafiltration of a protein product. In the literature, there is a...
08 September 2021 9,770 0 View
We are looking for experimental validation of the inhibition susceptibility of F1 sectors from different species. We have done an exhaustive literature search and are interested in data that, for...
01 July 2021 8,302 0 View