Pleas is it OK to select the primer sequence for Homosapiens Paraoxonase (PON1) gene to span only the exon-exon junction or leave it to no preference?

11 January 2024 0 6K Report

This is a hint from the NCBI database: This controls whether the primer should span an exon junction on your mRNA template. The option "Primer must span an exon-exon junction" will direct the program to return at least one primer (within a given primer pair) that spans an exon-exon junction. This is useful for limiting the amplification only to mRNA. You can also exclude such primers if you want to amplify mRNA as well as the corresponding genomic DNA.

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