I am working on a gene that has 14 variants, and I am specifically interested in knocking down one particular variant. After sequence alignment, I discovered that the variant I want to target has a unique sequence, but this sequence is also shared with four other variants. I am looking for strategies or techniques that would allow me to specifically knock down only this one variant without affecting the others.

Has anyone dealt with a similar challenge? What methods or approaches would you recommend for achieving variant-specific knockdown in this scenario? Are there any specific tools or design principles that could enhance the specificity of RNAi in such cases?

Any insights or shared experiences would be greatly appreciated!