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Hi everyone, I am currently working with the LUHMES cell line from ATCC and have run into a few issues. First, the protocol for culturing them involves coating plates with 50 µg/mL...
02 April 2024 9,183 0 View
I am trying to purify this eukaryotic protein (mol wt 60 kDa) in Rosetta strain. It is getting expressed in good quantity. However, there is another stubborn host protein (mol wt around 70 kDa)...
02 April 2024 4,051 3 View
For instance, using one hemisphere for brain slice staining via IHC and the other for analyzing neurotransmitter levels through LC-MS/MS? Are there any existing references or studies...
27 March 2024 2,503 1 View
I am learning the COMSOL software to study the band diagram of different photonic crystal structures. For a dielectric-based photonic crystal, only the k parameter sweep is needed to study the...
26 March 2024 3,631 1 View
I use TopSpin for the processing of NMR spectra (phasing, peak picking and integration) but I want to process the obtained data further in R. For this, I specifically want to get the signal...
22 March 2024 3,298 2 View
The added compost may not mineralized in the incorporation year. Hope it may mineralize fully in more than two years. So how to calculate the cost of compost for one (incorporation) or divide the...
21 March 2024 7,403 2 View
I am working with a DNA binding protein 180 Kda. after Heparin Purification or Gel filtration, when i used to centrifuge the purified protein at 10,000 xg, 10 mint, pellet is observed sometimes...
20 March 2024 6,068 4 View
The bulk-quantity 1D PANI nanowire/tubes were prepared as follows: analytical grade aniline (4 mmol) and tartaricacid (1 mmol) were dispersed in 10 mL of deionized water at room temperature with...
15 March 2024 4,427 2 View
Hello, I wanted to ask for help with protein modeling programs (not servers) with a free commercial license. thank you
14 March 2024 6,308 0 View
Can 0.5 mbar O2 oxidize polycrystalline Ag at room temperature, thermodynamically?
14 March 2024 1,454 3 View
In the journal entitled “The Transition from Gel Separatory Serum Tubes to Lithium Heparin Gel Tubes in the Clinical Laboratory”, how is turnaround time measured in the study, and how did the...
12 March 2024 1,958 1 View
Can the development of new technology help to save the natural environment and how the technologies use to reduce environmental pollution?
12 March 2024 4,831 2 View
Is it possible to grow graphene on the vertical wall of a structure, for example on the vertical walls of a SOI waveguide?
10 March 2024 2,178 2 View
I have fabricated chitosan acetate films and exploring their TS, Young's modulus and percent elongation/ductility. But they have shown a fluctuating trends. Generally it has been said that with...
08 March 2024 9,290 6 View
I want my synthesis to be performed at a particular reaction temperature say between 60-95 degrees. I am adopting Co-precipitation process and have two initial precursors. My general doubt is, if...
05 March 2024 3,531 3 View
What are the applications of cross-linked enzyme aggregates in the textile industry? How to reasonably control the particle size of the aggregate, so as to facilitate its diffusion into the...
26 February 2024 3,856 0 View
The position requires a working knowledge of standard optical testing techniques (interferometry, MTF, profilometry, etc), experience with optical instrument design and assembly, and basic...
26 February 2024 7,509 0 View
What is the particle size of the cross-linked enzyme aggregates? Can the prepared cross-linked enzyme aggregates be dissolved in water again?
24 February 2024 3,313 1 View
I am preparing SrCl2.6H20 samples in water for AAS analysis. By using the standard method if I make 1mg/ml solution it should be 1000ppm but with the AAS instrument, the measured absorbance comes...
21 February 2024 4,863 7 View
What is your suggestion for the best solvent for PGA polymer in food packaging?
20 February 2024 3,652 1 View
I have TiO2 powder form, and I want to use it for in vitro micropopulation. Please tell me how I prepare different concentrations of molarity and what solvent I should use for the preparation...
19 February 2024 4,790 3 View
I mixed 20 ml water with 10 ml DCM in funnel. I separated the organic phase and I analysed it with GCMS. The concentration is too low.
15 February 2024 7,759 5 View
What are some examples of successful learning analytics initiatives in higher education leadership?
15 February 2024 8,222 3 View
I recently got revision on my article where reviewer has given following comment "The calculation of bandgap is wrong please, do it again considering the baseline. The Figure is attached....
14 February 2024 7,160 0 View