Is the fluorescent protein must be totally photobleached in FRAP assay?

Hi I have tried to measure the mobility of a protein of interest by the FRAP assay. The protein was fused with GFP. I overexpressed the fusion protein in Hela cells. However, when the area of the...

14 December 2020 6,567 1 View

Why am I unable to coat oleate-capped NaCeF4:Gd,Tb nanoparticles with mesoporous silica shell?

My oleate-capped NaCeF4:Gd,Tb NPs have been synthesized via the thermal decomposition method. 1mmol of NPs has been dispersed in 20 ml of cyclohexane. I have tried dissolving 1 ml of NP in...

01 December 2020 4,713 2 View

Primer specificity check with primer blast individually or together?

Hi, When checking my designed primers for specificity, what is the difference if I run BLASTn for each primer individually versus together (ie. forward primer separated by 20 "N" then reverse...

03 November 2020 1,421 4 View

Sticky black, irregular patches in cell culture flask?

Hi all, Recently I encountered some irregular black/grey patches inside my cell culture flask (see attached image, x400 magnification). They are not motile, not floating, do not stick to cells...

08 July 2020 7,921 3 View

Transfected cells died after trypsinization ?

Hi all, I am facing a problem to trypsinize and combine/move the transfected cells into new well . I was planning to pool the transfected cells into a new well to ensure better cell-cell...

21 December 2019 9,692 5 View

Why I can't get the same result when I used two same light path configuration?

About a month ago,I built a light path that can be used to research ultrafast demagnetization on ferromagnetic film.But later,for some reason,I have to cleam my optical platform.And I recovered it...

31 October 2019 1,166 3 View

Problem with stable cell line generation?

Hi all, I am facing a problem to generate a stable cell line after successful transfection. I added puromycin to kill the untransfected cells, however, eventually all my transfected cells are...

19 September 2019 2,970 2 View

Co-transfection of miRNA mimics and inhibitors?

Hi, Is there any paper which reported the co-transfection of miRNA mimics and inhibitors? Thank you.

17 June 2019 1,087 3 View

MiRNA primer sequence?

Dear all, I have a miRNA primer sequence left by someone else and I would like to know about the sequence of this primer. Is there any method I can use to sequence and know the sequence of this...

14 June 2019 5,304 6 View

Qiagen or ThermoFisher Lipofectamine Transfection Reagent?

Hi all, I am planning for some transfection work involving a mammalian cancer cell line. Can I know the transfection kit from Qiagen or ThermoFisher lipofectamine is a better option? Do you...

23 March 2019 9,831 3 View

How can I calculate group index of one dimensional photonic crystal by using Rsof?

12 July 2020 6,040 2 View

How to measure polarization conversiong efficiency using RSoft?

Hello, I'm trying to measuring PCE by using RSoft. What I wanna measure is Polarization conversion efficiency that is defined as the fraction of the incident circularly polarized optical power...

11 December 2019 4,563 0 View

Can I do meta-analysis of some of the included studies and systematically review the rest?

Hi, For example: If I have 20 eligible studies (Both case-control studies and cross-sectional studies without any control group ) coming out of a systematic search. Can I do meta-analysis with 10...

26 March 2019 6,690 6 View

Can I do meta-analysis of some of the included studies and systematically review the rest?

Hi, For example: If I have 20 eligible studies (Both case-control studies and cross-sectional studies without any control group ) coming out of a systematic search. Can I do meta-analysis with 10...

02 March 2019 4,372 7 View

Bragg grating on optical waveguide?

Which software is better to design a bragg gratings on a waveguide depending on computational time and ease of design? COMSOL or RSoft Beam Prop? and Why? Can anyone suggest?

03 April 2018 1,029 0 View

How can i influence the kerr effect in Rsoft software?

Hello to all, I am trying to simulate a logic gate in Rsoft software.To achieve the best answer,I need to influence the kerr effect on my design,but i dont know where i can influence it?

11 December 2017 1,452 0 View

How to measure intensity of input or output port port using RSOFT?

21 August 2017 6,029 2 View

How to measure intensity of input or output port port using RSOFT?

07 August 2017 4,065 2 View

Is Rsoft software manipulate structures with multi input? if yes how to make more than one source?

Is Rsoft software manipulate structures with multi input? if yes how to make more than one source?

03 April 2017 6,100 0 View

Rsoft Fullwave simulation ?

Hi, I just learn to use Rsoft recently. I did a simulation for Y-spliter rib waveguide structure. My simulation is in 3D. When I launched the fundamental mode with continuous wave excitation type...

06 July 2016 8,407 2 View