Hello,
How would I i design a accuracy study for dynamic light scattering?
With a bead control and do different dilutions, and then calculate the % recovery?
I would appreciate if you can guide me on this. Thank you
Shei S.
There may be thermal (large in size) or small quantum fluctuations in the buffer (water solution). They disappear after filtration. Therefore, filter the buffer, and then do the experiment. This is what the manufacturers of the DLS device will recommend you. They believe that these are solid particles. However, this fluctuation can help you understand the experiment. Then you should think about filtering or not filtering. Read about fluctuations in water
Article Structure of Micelles of Sodium Dodecyl Sulphate in Water: a...
Preprint Nuclear quantum effect in aqueous micellar surfactant solutions
Ive done about 5 years of research on mineral growth where I used a significant amount of DLS. To determine whether the accuracy of the measurements is good/bad using the % recovery, depends on the bead size, (v/v%) concentration. Also, do you expect changes oin the beads due to the buffer over time? If so, on what time frame? Temporal variations complicates the interpretation of the results greatly and requires a solid foundation for the chosen instrument settings in my experience.
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