Can anyone suggest how to detect E coli without using any molecular methods in less than 4-5 hrs? I am trying to differentiate both pathogenic and non pathogenic E.Coli species.
Hi Dear,
you can detect E. coli without using any molecular methods in less than 4-5 hrs. These methods include temperature, immunomagnetic separation and ATP bioluminescence, paper strip etc. Articles below may help you.
Please let me know if the Articles are helpful for you.
Regards
Hossain
Dear Mohammed Sir,
Thank you for your response.These articles were so helpful for me.
thank you again.
You are welcome. I am happy to know that the articles above were helpful for you. Please let me know for further assistance.
Hossain
You can use IMS with antibodies unique to E. coli strain & then detect them.
See https://www.researchgate.net/publication/277131407_Highly_Sensitive_Bacteria_Quantification_Using_Immunomagnetic_Separation_and_Electrochemical_Detection_of_Guanine-Labeled_Secondary_Beads
Article Highly Sensitive Bacteria Quantification Using Immunomagneti...
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