It is known A. baumannii complex is implicated in HAI especially among intensive care patients. Typing the bacteria do help in proper infection control implementation.
Till date two techniques are known to resolve the complex, namely pulsed-field gel electrophoresis (PFGE) and 16s rRNA sequencing. Furthere details you can find in following literature
Schwartz DC, Cantor CR. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984;37:67–75.
Misbah S, Hassan H, Yusof MY, et al. Genomic species identification of Acinetobacter of clinical isolates by 16S rDNA sequencing. Singapore Med J. 2005;46:461–464.
Thank you ViVek Morya. I did find your answer helpful. Here we are planning to us PFGE (pulse Net) for the typing.
See the following PFGE vs Trilocus PCR vs MLST
Warner WA, Kuang SN, Hernandez R, Chong MC, Ewing PJ, Fleischer J, Meng J, Chu S, Terashita D, English L, Chen W, Xu HH. Molecular characterization and antimicrobial susceptibility of Acinetobacter baumannii isolates obtained from two hospital outbreaks in Los Angeles County, California, USA. BMC Infect Dis. 2016 May 4;16(1):194.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857389/
Dear Jolio. Ceasr and Mathew Arduino, I do appreciate your kind response to my question, the best methodology in molecular typing of A. baumannii.
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