I want to prepare media for cell culture use. Hence, I need to get water sterilized and add sodium bicarbonate and sodium pyruvate to it post-sterilization. I tried a different method, where I added sodium pyruvate and sodium bicarbonate to water and kept it for autoclaving. later I was able to see precipitates in the solution. Can anyone suggest what chemical reaction might have taken place. I found few articles suggesting the release of CO2 from sodium bicarbonate by autoclaving. But the precipitate formation has not been mentioned anywhere. Please suggest me what can be the error from my end.
@ Sabia, after autoclaving of sodium bicarbonate carbon dioxide is lost and sodium carbonate is formed which is very alkaline in reaction. Probably you have iron in the media which instantly precipitates in presence of sodium carbonate.
It it's not iron, could it be calcium? In other words, how pure are the water and reagents?
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