Need suggestion on ~500kDa protein?

10 January 2023 1 6K Report

I'm trying to get the 463kDa protein on 4-20% precast Tris-Gycine Gel. Transfer for 2 hours at 300mAmps, but getting a faint band. Suggestion, please!

Federico Svarc

Why you don't try separation in an adequate Sephadex column?

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