I'm trying to get the 463kDa protein on 4-20% precast Tris-Gycine Gel. Transfer for 2 hours at 300mAmps, but getting a faint band. Suggestion, please!
Why you don't try separation in an adequate Sephadex column?
check the plagiarism for the file attach below
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https://docs.google.com/document/d/17lS-gLLuADl3ZB2ayffPZYgqxVgkii8GvYToVELi1hA/edit?usp=sharing
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I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
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