Hi everyone,
I've been seeding and culturing fibroblasts onto my 3D-printed log-pile scaffolds to confirm their cytocompatibility. After I run a LIVE/DEAD assay, I observed promising cell viability in between the logs, inside the pores, and even the sides of the logs. However, I observe substantial cell death at the top of the logs. I'm not too sure what I'm doing wrong, especially since I get good viability everywhere else throughout the construct.
May someone please give me suggestions. Do I need to add more media to the scaffolds (I typically use 600 microliters)? Do I need to properly anchor the scaffolds in the well plates (I'm using a 48 well plate) to prevent any potential floating in media while in culture? Any suggestions will be greatly appreciated. Thanks!
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