We routinely get between 50 and 150 grams of adipose tissue that we process for stromal vascular cells - we currently mince by hand with scissors, but it's a huge time drain and takes a long time, which may be affecting cell viability. Does anyone know of any devices to help in this process? We are considering anything and everything, from a kitchen blender to expensive tissue homogenizers.
A high quality hand cranked rotary cheese grater works wonders for mincing tissue for subsequent enzymatic disaggregation. If it says it's dishwasher safe, it is also autoclavable.
http://www.williams-sonoma.com/products/2058758/?catalogId=21&sku=2058758&cm_ven=Google_PLA&cm_cat=Shopping&cm_pla=default&cm_ite=default&gclid=CN6UwNzy8MMCFYk2gQodZ44AUg&kwid=productads-plaid^89235667063-sku^2058758-adType^PLA-device^c-adid^45326111983
Ah, we're aiming to do cell culture, so freezing isn't an option, unfortunately. Thanks though.
I am afraid that might be the case... just figured I'd do some searching. My arm muscles are eager to find another answer!
Maybe something simple like this: http://www.target.com/p/as-seen-on-tv-vidalia-chop-wizard/-/A-10397839#prodSlot=medium_1_1 or a curved blade like: http://www.target.com/p/joseph-joseph-mezzaluna-folding-herb-chopper-with-integrated-blade-guard-green/-/A-16192474#prodSlot=medium_1_8
Something to speed it up a bit!
Ooh, I'm clicking around Target's site now - lots of good options! Non-scientific equipment makes me a bit nervous, but I imagine I could soak these in bleach then scrub. Thanks Kimberly!
I have found mincing mouse adipose tissue using scalpel blades with holders in ice cold buffer in petri dishes to be relatively easier than use of scissors. I'm afraid use of any machine which acts fast would only end up heating the tissue and decrease its viabilty for cell culture.To increase viability, you could keep the pending tissue immersed in ice cold buffer. Hope this helps!!
I also use scalpel blades. I place the adipose tissue into the lid of a petri dish and mince it it with 2 scalpel blades, one in each hand. The scalpel blades are sterile and everything is done at room temperature. I normally find that the procedure doesn't take too long and I generally get good cell recovery and viability.
A high quality hand cranked rotary cheese grater works wonders for mincing tissue for subsequent enzymatic disaggregation. If it says it's dishwasher safe, it is also autoclavable.
http://www.williams-sonoma.com/products/2058758/?catalogId=21&sku=2058758&cm_ven=Google_PLA&cm_cat=Shopping&cm_pla=default&cm_ite=default&gclid=CN6UwNzy8MMCFYk2gQodZ44AUg&kwid=productads-plaid^89235667063-sku^2058758-adType^PLA-device^c-adid^45326111983
Krishna, we haven't - I can't say I'm familiar. Can you tell me a bit about it?
Carl, what kinds of tissue have you used the cheese grater on? This is one of the options we are considering.
Have you heard of the gentleMACS?
It is meant for smaller tissue pieces (up to 1g) but can process 8 samples at once and is great for preserving cellular viability. It also has an kit for adipose.
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