Infectious Diseases

Infectious diseases, also known as contagious diseases or transmissible diseases, and include communicable diseases, comprise clinically evident... | Review and cite INFECTIOUS DISEASES protocol, troubleshooting and other methodology information | Contact experts in INFECTIOUS DISEASES to get answers

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Questions related to Infectious Diseases

How to inactivate lactoperoxydase without denaturating immunoglobulins in milk (no heat treatment)?

We would like to inactivate lactoperoxydase without denaturating immunoglobulins in milk. Heat treatment does successfully but denaturate proteins. What molecule or reagent, or substance we could...

17 May 2024 7,470 3 View

Is it possible to use a antibody specific for IF in IHC? and if yes, which will be the protocol??

I have an antibody specific just for ICC and WB but I should use the same antibody to perform an IHC staining. Is it possible to use this ab also in IHC?

16 May 2024 6,435 3 View

How can i use RFLP Method for these SNPs ARID5B rs4948496, DHFR rs1650723?

these SNPs do not have restriction enzymes is it possible to do RFLP

16 May 2024 8,010 1 View

How to improve 260/230 ratio in rna extraction for PCR?

The 260/280 ratio is ok between 1.8 and 2.0 however we were unable to advance due to the 260/230 ratio

14 May 2024 7,708 3 View

What is the reason for the color of the Agrobacterium cell pellet? Is it a normal cell color, or could it indicate contamination?

Strain: LBA4404 Growing media: YEB (pH 7) Temperature: 28°C, 200 rpm (overnight culture) Antibiotic use: Kanamycin and Streptomycin

14 May 2024 7,782 2 View

What educational initiatives are available to healthcare professionals to improve antibiotic prescribing practices?

Optimizing Antibiotic Use: The Role of Antimicrobial Steward... Optimizing Antibiotic Use: Strategies and Challenges in Anti... What educational initiatives are available to healthcare...

13 May 2024 6,562 0 View

What are the key objectives of antimicrobial stewardship programs, and how do they contribute to improved patient outcomes?

Optimizing Antibiotic Use: The Role of Antimicrobial Steward... Optimizing Antibiotic Use: Strategies and Challenges in Anti... What are the key objectives of antimicrobial stewardship programs,...

13 May 2024 9,810 0 View

How can healthcare organizations measure the effectiveness of their antimicrobial stewardship efforts?

Optimizing Antibiotic Use: The Role of Antimicrobial Steward... Optimizing Antibiotic Use: Strategies and Challenges in Anti... How can healthcare organizations measure the effectiveness of...

13 May 2024 7,786 2 View

What challenges do healthcare teams face in implementing effective antimicrobial stewardship practices?

Optimizing Antibiotic Use: The Role of Antimicrobial Steward... Optimizing Antibiotic Use: Strategies and Challenges in Anti... What challenges do healthcare teams face in implementing effective...

13 May 2024 4,199 0 View

How do antimicrobial stewardship programs balance the need for effective antibiotic therapy with concerns about overuse and antimicrobial resistanc?

Optimizing Antibiotic Use: The Role of Antimicrobial Steward... Optimizing Antibiotic Use: Strategies and Challenges in Anti... How do antimicrobial stewardship programs balance the need for...

13 May 2024 7,293 1 View

Can you explain the difference observed between my PFGE and WGS results for E. coli comparison?

Recently i did PFGE and WGS comparison with the same set of E coli strains. In my mind, if PFGE give two different pulsotypes, it is sure that strains are not the same. But when i did WGS, i...

13 May 2024 8,181 2 View

How does interprofessional collaboration enhance the success of antimicrobial stewardship initiatives?

Optimizing Antibiotic Use: The Role of Antimicrobial Steward... Optimizing Antibiotic Use: Strategies and Challenges in Anti... How does interprofessional collaboration enhance the success of...

13 May 2024 4,971 0 View

How does clozapine poisoning present clinically, and what steps are taken in its management?

Optimizing Clozapine Therapy: Overcoming Challenges and Enha... How does clozapine poisoning present clinically, and what steps are taken in its management?

13 May 2024 9,719 1 View

Are you interested to be co-author in different researches?

Dear Scholars, We are looking for co-authors in following fields to publish research articles. 1. Antimicrobial resistance 2. Public health 3. Maternal and Child Health Best regards

12 May 2024 675 28 View

Why do we think that women have the same sex drive as men?

Most guys feel like they are always the initiator and that sets up disequilibrium on the passion scale in the relationship. (Les Parrot)

10 May 2024 1,773 1 View

Why does an antibody work for IHC but not IF?

I have two antibodies that are staining well in IHC but not in IF for tissue sections, when using the same concentration and antigen retrieval method for both. I also know the secondary works well.

09 May 2024 3,976 4 View

Why are the mice in my control group mildly hyperglycaemic?

I am currently working towards developing a model for type 2 diabetes, using fructose and streptozotocin (STZ). Prior to injecting my C57BL/6 mice with STZ, we tested blood glucose with a...

09 May 2024 4,005 2 View

Can you discuss the economic implications of dexmedetomidine use and any strategies for optimizing its cost-effectiveness in healthcare settings?

Can you discuss the economic implications of dexmedetomidine use and any strategies for optimizing its cost-effectiveness in healthcare settings?Dexmedetomidine: A Comprehensive Review of...

09 May 2024 6,834 1 View

How to isolated bacteriophage with lytic activity from water and other samples?

My student is isolating phages against ESKAPE pathogens, but she is getting a minute plaque. Can you please suggest how to get better results?

08 May 2024 9,643 2 View

What are Trends in viral infection among cancer patients?

Hello every one Can any one suggest me trends subjects in viral infection in cancer patients for PHD thesis? Thanks in advance.

06 May 2024 5,302 3 View

Can antigen retrieval protocol work for IHC/IF for formalin fixed tissues fixed for more than 7 days or even 2-3 months ?

I want to ask if antigen retrieval process works for formalin fixed tissues of more than 7 days or even 2-3 months ? Or it should be done for tissues that are fixed for max upto 24 hours ?

06 May 2024 2,450 2 View

Size acrylamide pores?

Good evening, I was wondering what is the size of Acrylamide/Bis-Acrylamide in a PAGE 4%/8%. I'm asking because after the run I found that some of the sample stayed in the well

05 May 2024 1,626 3 View

Recombinant protein purification?

Dear everyone, I am currently making a recombinant protein (protease) for checking its activity by zymography. However, before column purification (his-tag), the activity was shown only one band...

05 May 2024 1,243 3 View

Protein purification for his tagged protein?

pI of my protein is 4.1. I am using Ni coumn to purify it . Which binding buffers will be good? Even the pI is low can it still bind to nucleic acid? How do I know?

05 May 2024 8,587 2 View

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