I am currently working towards developing a model for type 2 diabetes, using fructose and streptozotocin (STZ). Prior to injecting my C57BL/6 mice with STZ, we tested blood glucose with a tail-prick, using an AccuChek Instant glucometer. As per methods used in relevant literature, I fasted my mice for 4 hours prior to checking blood glucose (BG). The mean BG in my normal control, which did not receive fructose water was around 8 mmol/L, a BG akin to mild hyperglycaemia. Bear in mind, this group is not due for induction, and received a normal diet of commercially available mice pellets. For the second BG test, I fasted my animals for 6 hours, and got the same if not similar results.
Please assist.
May the commerically diet cause hyperglycemia which have high content of sugars ,Stress
Type of diet ,The weather of region ,The breeds of mice and may be you dont islate the control from the treatment groups
Stress and excess sugar with tea.insullin more, starvation , alcohol, movement of body is less than more calories, physical activities less, sleeping not properly so many causes.
Unanimous food habits,.if sugar levels mor
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