How can i use RFLP Method for these SNPs ARID5B rs4948496, DHFR rs1650723?

Leena Pavitha @Leena-Pavitha

16 May 2024 1 8K Report

these SNPs do not have restriction enzymes is it possible to do RFLP

Paul Rutland

Almost certainly yes.

The base change itself may not create/delete an enzyme cut site for rflp but using dcaps

http://helix.wustl.edu/dcaps/dcaps.html

it will be seen that a second deliberately introduced base change in the amplifying primer will almost always create a restriction site specific to the base found in the template sequence.. This software designs primers to detect either/both of the bases in the snp position. You just have to be a bit careful that the cut site that you are creating does not occur naturally within the amplimer making analysis more difficult

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