In acrylamide gel electrophoresis, the upper gel is only a means for the movement of proteins and the separation of waste materials with it.

The lower gel is selected based on the molecular weight of the desired protein, and the smaller the protein, the higher the gel percentage, and vice versa. As the gel percentage increases, the pores and holes in the gel will also become smaller.

Thank you for your answer. I know that, I just wanna have an idea of what the diameter of the pores can be

I suggest you the use of a gradient of poliacrylamide concentration!

eGood look.

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