Genetic Analysis

Genetic analysis can be used generally to describe methods both used in and resulting from the sciences of genetics and molecular biology, or to... | Contact experts in Genetic Analysis to get answers

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Questions related to Genetic Analysis

How to determine the genotype of a sub-haplotype in heterozygote patient??

Hi everyone, I want to determine the real genotype of my SNP in heterozygote patient but I can’t? Should I do the linkage disequilibrium analysis, if yes, please tell me how? I am new in SNP...

03 September 2018 7,146 2 View

Significance of repeat runs in umbrella sampling ?

Statistically, Is there any need of repeat runs in umbrella sampling simulations as well as simulation where outcome obtained from independent systems and still follows particular trend/path/curve.

12 August 2018 2,319 0 View

Experiences on microsat genotyping with MiSeq?

So far we’ve been doing microsatellite analyses with a capillary sequencer. The machine is getting old and we need to replace it. Since we’re regularly using MiSeq or HiSeq for other approaches...

28 June 2018 3,623 5 View

What is the optimum time of incubation to label DEPE-PEG-Biotin to membrane of exosome?

I want to label exosomal membrane with DSPE-PEG-Biotin in order to isolate magnetically with SA-IONP. Does anyone has any suggestions?

03 June 2018 8,157 0 View

How much protons can every proton pump pump per second?

How much protons can every proton pump pump per second? How about other ions pump?

01 June 2018 2,722 1 View

Can anyone assist with a PCR protocol using fluorescent labelled primers for microsatellite genotyping? Or is it the same as a normal PCR reation?

I'm working on genetic diversity of cattle breeds. For that I'm using microsatellite markers. I just want to know if PCR reaction is performed differently when using a fluorescent labelled...

22 May 2018 9,594 8 View

Dear all, I am trying to implement microsat data to RDA analysis but I am not sure how exactly to do it. Any help/comments?

I am working with three aquatic plant species occuring in island-like habitat (small water bodies) scattered in an intensive agricultural landscape. My main question is to see if there are genetic...

12 April 2018 8,908 7 View

Should one remove full- or half-siblings prior to landscape genetics analyses?

Hello, I am preparing to run an individual-based landscape genetics analysis on Eastern Indigo Snakes and was wondering if someone could provide some guidance on how to deal with individuals...

04 April 2018 8,741 3 View

Molecular biology is used to produce GMOs. What are your views on GMOs?

A genetically modified organism (GMO) is any organism whose genetic material has been altered using genetic engineering techniques (i.e., a genetically engineered organism).

23 February 2018 1,309 11 View

Are deuterated solvents necessary for 13C NMR?

I want to do a metabolite carbon-tracing experiment, using glucose labeled with 2-13C and will measure it with 13C NMR. Do I need to use a deuterated solvent if I don't care at all about what the...

16 January 2018 4,686 5 View

LPS genetic analysis variance between samples?

Hello everybody, i am trying to characterise my LPS model (rat) with qPCR analysis to check the level of neuroinflammation, but i have a pretty big variance between the LPS treated...

13 January 2018 4,809 2 View

Could anyone explain why some Arabidopsis genes are present as single copy in Brassica napus genome?

I am trying to identify and elucidate the function of a gene (let say X) in Brassica napus that control flower development in Arabidopsis. Using the sequence of Arabidopsis X, I conducted a...

10 December 2017 7,782 2 View

How fresh do ruminant fecal samples need to be for next-generation DNA sequencing to determine diet composition?

I need to determine the diet composition and relative proportion of plant species in mule deer diet. What I currently understand is that fecal samples need to be fresh in order to accurately...

27 September 2017 3,997 1 View

Input file FSTAT for partial mantel test?

How do I prepare data file for FSTAT software for mantel partial analyzes? I have microsatellite marker.

06 September 2017 1,739 5 View

Have you COI sequences of Potamotrygon orbigyi?

Hello, I´m including P. orbignyi in a population analysis for conservation genetic study. In GenBank are a few sequences; but, into them, there is not any sequence from Brazil, where was...

25 August 2017 6,778 2 View

Annealing time or annealing temperature which one is better for PCR optimization ? and why?

Hello researchers the annealing temperature gradient is the routine step in PCR optimization , but why we do not use annealing time for optimization? regards .

21 August 2017 7,221 14 View

Question about lncRNA-miRNA interaction

Hi everyone, I have a question about How we can predict miRNA binding sites on lncRNAs. Some papers recommend generally some websites such as miRanda, TargetScan or PicTar, but I think none of...

15 July 2017 5,954 3 View

Glycerol or Paraffin oil, which is better for bacteria preservation?

I am exploring options to preserve bacteria namely, Staph. aureus, E. coli, P. aeruginosa and Salmonella typhi. I want to be able to preserve them for a period of 1 year and will be using them for...

07 June 2017 2,277 8 View

How calculate genetic correlation and phenotypic correlation?

If I have data of performance of chickens, biochemical analysis, egg production traits How calculate genetic correlation and phenotypic correlation?

24 May 2017 4,147 10 View

Residence time from potential of mean force-stable state picture

Hi all, To calculate residence time from potential of mean force (PMF), we use stable state picture. Here a reaction state, product state are defined. This is done from radial...

24 May 2017 8,689 1 View

Does anyone know how exactly fluctuates âr ? (i.e. interindividual rousset's distance, Rousset 2000). Are there thresholds like fst values ?

Hi everybody, I would want to describe my figure showing pairwise genetic differentiation between Vipera berus individuals against logarithm of distance. More precisely, i would want to know how...

12 March 2017 4,237 4 View

Detection of antisense long non-coding RNA. Does someone have experience performing qRT-PCR to detect antisense long non-coding RNA?

Hi research fellows, does someone have experience performing qRT-PCR to detect antisense long non-coding RNA? We have isolated RNA from human cells and would need a protocol to transcribe RNA into...

27 February 2017 9,712 6 View

Best program to measure contemporary (20 - 40 generations) migration/ gene flow?

I want to look at the level of gene flow / migration between fish populations sampled at 10 km intervals within a river system to see if there is a link between genetic and morphological variation...

23 February 2017 2,131 5 View

Methylation, SNP array and clinical dataset for ovarian cancer beside TCGA?

May be anyone can help us with the the information for the database having for methylation, SNP array and clinical dataset besides TCGA "Ovarian serous adenocarcinoma". Database with only SNP...

22 February 2017 1,794 1 View

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