There is two types for AFLP markers, we can use single restriction and PCR primer or we can use two type of restriction enzymes (primers).

Dear Yadaw

Restriction fragments of the genomic DNA are produced by using two different restriction enzymes: a frequent cutter (the four-base restriction enzyme MseI) and a rare cutter (the six-base restriction enzyme EcoRI). The frequent cutter serves to generate small fragments, which amplify well and which have the optimal size range for separation on a sequence gel, whereas the rare cutter limits the number of fragments to be amplified.

By using two enzymes you get enough (size-) polymorphic fragments that are still small enough to amplify and to detect them on a scanner in a large number.

Dear Prof Seied Mehdi Miri ,

Can you send this reprint

t8.pdf

1.25 MBto my mail

[email protected]

Dear Dr. Rao

If you click on the t8.pdf file, you can download the file.

Thank you to all for answering.