I think the DNA is the better one and easy too

Ishag i cant extract the DNA, have to take the smear positive sputum to do culture first and then genenitic analysis?

Hi Abdifetah,

You can transport TB samples out of a country but it does require a lot of paperwork.

This becomes easier if you are able to inactivate the samples in some way, so may be killing them with heat or storing them in a special buffer. Although if you need to perform culture, I do not think this will work for you.

Each country has different regulations and laws as to how biological samples must be transported and how samples are classed (biological specimen vs. clinical sample). You will need to look this up for your specific work.

You may then require an export license from the government to remove them from the country, there may also be paperwork required to get them through customs at the destination country.

You will also need to find a courier service which is able to deal with these sorts of samples. I think most international courier firms should be able to handle dangerous samples such as TB sputum.

Hope this is helpful,

Eleanor

Thank you Eleanor may Townsend for ur valuable comments.

As Eleanor says inactivation of samples is key. If samples are not infectious, not harmful, contains no living organism, and no viable virus, etc. you are much more probable to get them through customs. For shipping infectious substances, UN 2814, UN 3373, rigid rules apply, also on packaging.

Hi Dr abdifatah i hope you are doing well.

1. If you are traveling more than 12 hours use sputum preservitive called cetylpyridinium chloride (CPC). first you treat the sample with 1% CPC (1:1 CPC and sample), mix until an even solution was observed, then incubate at 25 °C for 7, 14, or 21 days

2. If transport times are less than 12 hours, even specimens without CPC can be transported at room temperature.

Mahadsanid

Abdifetah Omar

Thank u walaal shaafici for ur information

U wlc bro abdifatah