Genetic Analysis

Genetic analysis can be used generally to describe methods both used in and resulting from the sciences of genetics and molecular biology, or to... | Contact experts in Genetic Analysis to get answers

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Questions related to Genetic Analysis

Is there any method to isolate genomic DNA of a gene?

I have isolated full length cDNa of squalene synthase gene from root of tongkat ali. however I would like to validate my result whether the gene have intron or not. so I would like to isolate the...

27 February 2015 7,687 5 View

How to use the NCBI GEO analysis/data-sets to find out expression profile of a particular gene?

In research articles people often show meta analysis of a gene using NCBI GEO data sets or analysis, i want to know the procedure by which i can derive the information for a particular gene?

22 February 2015 1,188 4 View

How strict is the sequence of leader-peptides?

Dear all, I want to express my protein in the periplasm of E. coli and decided to use the common pelB-leader-sequence for translocation to the periplasmic space. Now I also want to experiment with...

09 February 2015 3,783 4 View

Does the rbcL gene contain introns?

It appears that the large subunit of the ribulose-bisphosphate carboxylase gene (rbcL) does not contain introns, at least not in the species I have checked (e.g., Camellia impressinervis; see...

05 February 2015 7,460 3 View

How to use the NCBI GEO analysis/data-sets to find out expression profile of a particular gene?

In research articles people often show meta analysis of a gene using NCBI GEO data sets or analysis, i want to know the procedure by which i can derive the information for a particular gene?

02 February 2015 2,522 3 View

Does the rbcL gene contain introns?

It appears that the large subunit of the ribulose-bisphosphate carboxylase gene (rbcL) does not contain introns, at least not in the species I have checked (e.g., Camellia impressinervis; see...

02 February 2015 4,539 4 View

Does anyone have hLRP6 in pDonr221?

I am looking for human LRP6 in a pDonr221 vector (or any other vector compatible with Gateway LR Recombination). Does anybody have it and is ready to share a little bit of it? Do you also have a...

02 February 2015 4,733 3 View

Do you have any suggestion on papers comparing average Fst and heterozygosity in several cattle breeds using dense SNP chips?

This kind of comparison seems tricky and polemic, however, still very necessary!

28 January 2015 2,926 4 View

Why does Arlequin give results in two digits, instead of five digits as it usually does?

Why does Arlequin give results in two digits, instead of five digits as it usually does? In the same database, other loci calculations are expressed with five digits (i.e. 0.02513) but for one...

23 January 2015 1,003 2 View

Which are the limitations of non-invasive prenatal diagnosis using Fetal Cells Circulating in Maternal Blood?

Fetal cells circulating in the peripheral blood of preg-nant women are a potential target for noninvasivegenetic analyses. They include epithelial (trophoblas-tic) cells, which are larger than...

21 January 2015 4,106 9 View

NGS Analysis: How can I detect the common variants in two VCF files?

NGS Analysis: I am working on WES data of two patients in one family. How can I detect the common variants in the two VCF files?

19 January 2015 7,708 3 View

MEGA 6: Treatment of Gaps/Missing Data - What to choose?

I had difficulty in amplifying DNA stretches due to sub-optimal insect specimens and now have multiple sequences of varying lengths produced from a variety of primer pairs. All do, however, fall...

19 January 2015 4,921 9 View

How can I see exons and introns sequence in vector NTI or another software or database website?

I want see exons and introns sequences in my genes but these gene are for Capra hircus and too many databases have not this organism's genome, for example Ensembl or USSC database. How can I see...

15 January 2015 2,971 3 View

How can I calculate genetic gain in kg per year?

I took data on 30 varieties released from 1981 till 2013. Now I need to calculate breeding progress or genetic gain in kg per year.

12 January 2015 7,399 7 View

What is the role of histone and DNA modification enzymes in gene control and repetitive DNA in chromosome stability?

I cannot find the answers for the following of topics from molecular genetics . Topic Genes to Genomes. X inacttivation/The role of histone and DNA modification enzymes in gene...

11 January 2015 8,020 0 View

How does one explain the increased message level of an exon containing a TALENs-induced frameshift mutation?

We introduced a 16 base deletion via TALENs in the 1st coding exon of a gene (exon 4) which caused a frameshift leading to a premature stop codon. We just ran qPCR using two primer sets on brain...

01 January 2015 4,575 9 View

Denovo chimera removal is recomended or not?

during the analysis of V3 region of 16S Next gene consensus. should I remove denovo chimera or proceed for clustering (OTU formation) without chimera? what should be the minimum % of identity (90,...

01 January 2015 3,137 2 View

Which are the limitations of non-invasive prenatal diagnosis using Fetal Cells Circulating in Maternal Blood?

Fetal cells circulating in the peripheral blood of preg-nant women are a potential target for noninvasivegenetic analyses. They include epithelial (trophoblas-tic) cells, which are larger than...

01 January 2015 3,518 10 View

NGS Analysis: How can I detect the common variants in two VCF files?

NGS Analysis: I am working on WES data of two patients in one family. How can I detect the common variants in the two VCF files?

01 January 2015 4,933 4 View

How can I see exons and introns sequence in vector NTI or another software or database website?

I want see exons and introns sequences in my genes but these gene are for Capra hircus and too many databases have not this organism's genome, for example Ensembl or USSC database. How can I see...

01 January 2015 515 3 View

Why do I get very low LOD scores from linkage disequilibrium analysis in haploview?

I'm doing some linkage disequilibrum analysis between SNPs which I expect to be showing high levels of disequilibrium. Although the D values haploview is giving me are high the LOD values are...

01 January 2015 268 2 View

Why does Arlequin give results in two digits, instead of five digits as it usually does?

Why does Arlequin give results in two digits, instead of five digits as it usually does? In the same database, other loci calculations are expressed with five digits (i.e. 0.02513) but for one...

01 January 2015 9,403 2 View

What is the role of histone and DNA modification enzymes in gene control and repetitive DNA in chromosome stability?

I cannot find the answers for the following of topics from molecular genetics . Topic Genes to Genomes. X inacttivation/The role of histone and DNA modification enzymes in gene...

01 January 2015 2,865 2 View

How can one remove EDTA from low yield/ low MW DNA extracts?

I have extracted historic DNA from animal tissue using the Qiagen DNeasy kit and eluted the DNA in the provided elution buffer, which contains EDTA. I will perform whole genome sequencing on an...

01 January 2015 6,775 4 View

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