Hello everybody,
i am trying to characterise my LPS model (rat) with qPCR analysis to check the level of neuroinflammation, but i have a pretty big variance between the LPS treated animals.
excluding the differences between animals and how the may naturally deal with inflammation, do you think that specifically LPS may act so differently into animals and induce so different responses?
cheers!
Even if stereotactical Injections are the same, there may be anatomical differences between animals that will affect distribution. Imaging during injections may help.
You should clarify first if it is direct injection into the brain, peripheral ip injection, the dose, serotype and company. Then if you dissect brain regions, if you remove blood before tissue processing and how long you collect after LPS injection.
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