Where to find data set for big data analytics ?

I am looking for a data set (preferred to be spatial data) in order to apply big data analytics on it, can you help me to find such a data set please?

05 January 2021 4,860 4 View

How do I properly normalize spectra/peaks for comparison (Photoluminescence and Electroluminescence) ?

Hello, This questions pertains to comparing peaks from spectra of 2 or more different samples in a fair way through normalizing I know Origin normalizes based on the highest peak however if I...

12 December 2020 403 1 View

Can xylose be formed from fructose over acid catalysts?

I am new in the field of sugar chemistry. I wanted to know if it is possible for fructose to be converted to xylose over acidic conditions. I saw in some schemes a pathway from fructose to xylose...

07 December 2020 5,247 3 View

Why I obtain very high surface area under HMF peak in HPLC even with suitable dilution?

I am converting Fructose into HMF, Levulinic acid and formic acid are possible side products. I analyze the data via HPLC, bio rad column, I got nice results for some reaction set. But when I...

23 September 2020 6,375 7 View

What is so special about graphene?

Why is graphene so important?

18 September 2020 3,857 9 View

What is the difference between ultra-high vacuum chamber and edwards vacuum coating unit if we are using the the technique of thermal evaporation ?

developing thin films in ultra high vacuum chamber thermal evaporator.

09 September 2020 4,172 3 View

Can anyone help me out to find out my masters thesis research topic??

My area is software engineering, I haven't any idea about the thesis. need lots of help. I want that the topic should be Lil bit easy. :P Thank You

06 September 2020 4,678 14 View

What are the best topics for research on environmental informatics, immuno informatics and nano informatics now a days alongwith the best methodology?

best research topics and methodologies on pollulatnts which influence genetic diseases and the treatment through nano technology?

09 August 2020 5,326 1 View

How to extract features from an image and save these features as a file, do you have any python code?

My question is: I need to extract features from an image and save the features of each image in a separate file so that later on, I can apply arithmetic operations on these features. I need a...

05 July 2020 3,303 3 View

I need help with the interpretation of why not obtain results in real-time PCR?

Dears, I need help with the interpretation of why not obtain results in real-time PCR when I use Taq man SNP genotyping assay ID: rs 22756913 (IL17A) from Thermo fisher scientific where the...

23 June 2020 8,121 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View