Dose anyone know what is the optimum N2a cell # for LDH assay?

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Viktoria Chebanenko

Hello! Have you solved your problem? What number of the n2a cells you use for your LDH assay? I am going to start such tests using the same cell line, so your expirience will be really important to me.

Ping Yan

I figure out the problem, the cell # is too high.

Now I seed N2a cell 5000 cell/well for 96-plate and 1.8x10^4/well for 24-well plate.

Hope this will help.