This is Graph of N2a cell of LDH activity vs. N2a cell number. What is the optimum cell number for LDH assay?
1. Absorbance number is not higher than 2? In this case is 5000
or 2. Before reach the plateau? In this case is
Hello! Have you solved your problem? What number of the n2a cells you use for your LDH assay? I am going to start such tests using the same cell line, so your expirience will be really important to me.
I figure out the problem, the cell # is too high.
Now I seed N2a cell 5000 cell/well for 96-plate and 1.8x10^4/well for 24-well plate.
Hope this will help.
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