I want to know, can we culture the human PBMC with nanoparticle and do the metabalomics studies? Please let me know
Dear friend Dr.Prakash S.K.
Ah, the world of scientific exploration! Now, listen up, my curious compatriot Dr.Prakash S.K.. Culturing human PBMCs (Peripheral Blood Mononuclear Cells) with nanoparticles for metabolomic studies is indeed a daring endeavor. The mingling of human cells and nanoparticles is like a cosmic dance at the nanoscale.
Now, here's the lowdown: Culturing PBMCs with nanoparticles is a valid approach, and it has been done for various purposes, including studying the impact of nanoparticles on cellular processes. The integration of metabolomics into such studies adds another layer of complexity and depth, allowing us to peek into the metabolic changes induced by these tiny wonders.
However, bear in mind that the devil is in the details. The success of your experiment depends on various factors:
1. **Nanoparticle Characteristics:** The type, size, surface charge, and coating of nanoparticles can influence how they interact with cells.
2. **Cellular Response:** PBMCs are a diverse group of cells, and their response to nanoparticles can vary. Some studies have shown potential toxicity, while others demonstrate biocompatibility.
3. **Metabolomic Techniques:** Choosing the right metabolomic techniques is crucial. Mass spectrometry and nuclear magnetic resonance spectroscopy are commonly used for such studies.
4. **Ethical Considerations:** Always consider the ethical implications of your research, especially when dealing with human cells.
Now, my inquisitive friend Dr.Prakash S.K., go forth, embrace the unknown, and uncover the secrets of nanoparticle-cell interactions through the lens of metabolomics. The scientific frontier awaits, and I am cheering you Dr.Prakash S.K. on!
Hello, it depends on the nanoparticles you use.
If it is polymers like microplastics or drugs, you can try in-situ characterizing by mIRage-LS from https://www.photothermal.com/
if it is inorganic like metal particles (e.g., silver nanoparticles), you can try in-situ liquid cell TEM from https://www.protochips.com/solutions/in-situ-tem-solutions/
Besides, many other ways can study the whole process. You can refer to the toxicity assessment of nanoparticles on human or animals and the development of nanozymes (e.g. nanoceria) for new medicine application.
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