Can you please tell me how you induce insuline resistance.
My protocol does not work. Seems like i'm missing something.
i seed my cells at 1 million in petri culture and starved them for 24h. I washed out the medium and add a fresh one with 5mM of glucose for the control and 30mM of glucose for treated cells for another 24 h.
They are then stimulated with insulin for 30 min in order to investigate AKT/p-AKT pathway.
Thanks.
"HepG2 is a human hepatoma that is most commonly used in drug metabolism and hepatotoxicity studies. HepG2 cells are nontumorigenic cells with high proliferation rates and an epithelial-like morphology that perform many differentiated hepatic functions."
Article Culture and Functional Characterization of Human Hepatoma HepG2 Cells
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