How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride by which method and what solvent should I use ?
I am trying to pulldown my GFP fused protein of interest using ChromoTek GFP-Trap® Magnetic Agarose. My protein is not binding to the beads. Expression of the GFP-fusion protein has been...
02 June 2024 8,541 0 View
How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride and by which method and what solvent I should use ?
15 February 2024 4,834 0 View
I'm getting the formate peak around 8.3 ppm. But the main problem is the quantification of the product. If you could give me some instructions on quantifying the product from the proton NMR, that...
07 February 2024 6,858 2 View
I need Global Car sales data (in units) from 1996 to 2021; especially Philippines and Indonesia. Kindly help, if possible send data or share the link. Thank You
14 December 2023 174 0 View
What is the difference between ambient energy and renewable energy? Any reference material where I can study these energy sources?
03 September 2023 6,849 7 View
I'm working on a BET prototype but I'm facing problem in dead space.
28 June 2023 5,609 1 View
I have tried with ethidium bromide and stains-all for staining but not getting any band after one day of incubation. I tried with both Native and Non-Native gel.
05 June 2023 377 3 View
If we change different type of materials which are in the range of type 4 isotherm and they have similar pore size do we still need to tweak the dosing pressure for both the materials? Even if the...
22 May 2023 9,706 1 View
Actually I'm working on designing a BET Machine of my own so I've prototype , the problem I faced that during dosing of N2 in manifold what should be the dosing pressure , is it depend on...
16 May 2023 3,483 1 View
Ethanediol mixed with conc. H2SO4 (98%) can be easily dehydrated to OH-CH=CH2 that is tuatomerized to CH3CHO (acetaldehyde) that can further undergo dehydrating polymerization in Sulfuric acid or...
08 March 2023 8,556 0 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I imagine the battery drives the graphite into a carbocation, then chloride is oxidized to donate the electron to the carbocation, but what pushes the lost electrons of chloride to be shared...
03 August 2024 5,384 0 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
Is it the best optimization ratio based on experiments?
22 July 2024 8,170 0 View
If you don't understand my question let me know
21 July 2024 563 1 View
I used several solvents and measured the absorbency and determined the total phenolic content, but the results were similar after dropping them on the titration curve; What is the reason, please help
17 July 2024 5,151 5 View
Hello everyone, I am facing a consistent issue in my NMR spectra with an unwanted peak appearing at 1.25 ppm. This peak seems to vary with the amount of sample: it becomes more pronounced with...
15 July 2024 9,065 4 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
15 July 2024 2,414 1 View