Help me conduct a neuroscience experiment for my master's thesis?

22 January 2021 9,994 5 View

Continuous glucose monitoring in animals ?

Is continuous glucose monitoring, comparable to what is existing in humans, available for mice and/or rats without large surgery ? thank you !

21 September 2020 5,950 2 View

How to converge hyperelastic modeling with large rotations in Abaqus?

Hi, My problem is similar to simulate fold soks in 3D model with 2D elements (shell). I would like to use Membrane element but it is not possible for hyperelastic material in abaqus. I use S3 (or...

17 March 2020 795 5 View

How to properly calculate the mutational rate in bacteria?

Hello everyone, I hope y'all can answer a simple but problematic question. I have been doing mutation accumulation experiment in 3 parallel lineages in bacteria and I now want to calculate the...

05 December 2019 5,648 4 View

Does anyone know how to implement restricted permutations for multivariate analysis of split split plot experiments in R?

02 April 2019 4,257 1 View

Is there any effective transfection protocol for cells cultured in 3D hydrogels?

I would like to transfect micro-RNA mimics in 3D models. Lipofectamine RNAimax works well in monolayer cultures but I couldn't find convincing evidence it could work sufficiently well in 3D.

27 November 2018 4,524 3 View

How to design a low ecological footprint electric machine?

To my knowledge, electric machines (motors and generators), apart from their electronics, are made of at least two or more of these materials: Electric steel (stator and rotor iron...

08 November 2018 1,783 9 View

Quickly pellet floating fatty cells in culture plate?

Hi, I have fatty floating cells in culture medium and I need to simply/quickly pellet them without degrading cells nor removing themfrom the plate. Any idea ? thanks

21 March 2018 4,569 3 View

Help with NCBI table2asn

Does anyone have experience with the table2asn tool of NCBI? It is used for submitting annotations of genomes using GFF files. It can be found at this location:...

23 May 2017 1,696 3 View

What is the easiest way to determine the absorption spectrum of a bacterium?

We would like to determine the absortion spectrum of a bacterium. I have, however, never done something like that. It's to see if there are any absorption peaks present and if the bacterium would...

05 January 2017 6,933 11 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Which one carries more value? A research paper in a Scopus Indexed conference proceedings or the same paper in a Journal without Scopus indexing?

I am looking for the suggestion among which one to pick from the above two categories for a research work.

27 February 2021 10,025 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Which indexation is better Scopus or Web of Science?

Both are very repetitive indexing agencies.

26 February 2021 9,455 4 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View