Hi everyone,

I am currently working with mouse skin and lung tissues preserved in RNAlater (Invitrogen). However, I have limited experience with single-cell RNA sequencing (scRNAseq). I would like to ask if anyone has experience isolating single cells from RNAlater-fixed tissues. Specifically:

  • Is it feasible to isolate viable single cells from tissues fixed with RNAlater?
  • Can these fixed cells be successfully used for scRNAseq experiments?
  • I would greatly appreciate any advice, protocols, or insights you could share regarding this matter.

    Thank you so much for your support!

    Best regards, Thuong

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