Diagnostic Methods

Does applying the rule of thumb perform better in pest and disease early warning systems or statistical models?

Rule of thumb proves better for most instances and the predictions of statistical models are close to the real situation. Which one should be relied on the most?

03 March 2014 2,405 0 View

Seeking a commercial kit that can help us measure PPi in human serum. Is there a necessary special preparation of the sample prior to determination?

Anyone have experience in the determination of pyrophosphate in serum or plasma?

03 March 2014 2,901 0 View

How many microsporidia spores are needed in stool for sufficient DNA extraction?

I am trying to find a new way for DNA extraction of intestinal Microsporidia spp.

01 January 2014 3,428 3 View

What is the best source for RNA extraction: Blood or Serum in Blue tongue?

In a RT-PCR for diagnosis of blue tongue virus what is the best source of viral RNA blood or serum

01 January 2014 5,108 0 View

Why does Arcanobacterium haemolyticum grow better on sheep blood agar than horse blood agar?

I know it grows best on human blood but this is not usually available. Horse and sheep blood are often used in blood agar solutions, but what is it about the blood that makes one preferable to the...

11 November 2013 7,759 1 View

Why does Arcanobacterium haemolyticum grow better on sheep blood agar than horse blood agar?

I know it grows best on human blood but this is not usually available. Horse and sheep blood are often used in blood agar solutions, but what is it about the blood that makes one preferable to the...

07 November 2013 957 1 View

How can I get rid of non-specific amplification in a LAMP assay?

I am trying to develop a LAMP assay for a fungal plant pathogen using HNB as the indicator. However, I am getting amplification even in negative controls without any DNA template added. I have...

10 October 2013 4,823 15 View

How can I get rid of non-specific amplification in a LAMP assay?

I am trying to develop a LAMP assay for a fungal plant pathogen using HNB as the indicator. However, I am getting amplification even in negative controls without any DNA template added. I have...

01 October 2013 5,519 14 View

What is the best way to study Proteomics of Aspergillus Fumigatus and identify its novel immunoreactive proteins?

A.fumigatus has been found to be primary causative agent responsible for infectious diseases.

09 September 2013 9,525 5 View

Is it possible to run the ELISA test optimization without the positive control?

ELISA test were run to optimise the appropriate antigen concentration and serum dilution factor. I had triplicates of substrate control, negative control and blank for my analysis. Since this is a...

21 August 2013 9,811 6 View

Is it possible to run the ELISA test optimization without the positive control?

ELISA test were run to optimise the appropriate antigen concentration and serum dilution factor. I had triplicates of substrate control, negative control and blank for my analysis. Since this is a...

08 August 2013 9,446 7 View

Can someone explain my unexpected results from a serum creatinine assay and how to fix it?

I recently used a serum creatinine assay kit from abcam (link below) and obtained some unexpected results. I was wondering if anyone here could share their experience with measurement of serum...

01 January 2013 6,948 3 View

Dear Sueb Sueb and your colleagues! Thank you for interest to our project < Express method ....> ?

Unfortunately due to the termination of the financing in our country this and other works the project was stopped. We are looking for sponsors to decide this problem…. The main direction of our...

01 January 1970 249 0 View

Hypothesis:   Non-invasive method to detect thyroid nodule malignancy: Near-infrared Spectroscopy (NIRS)

Near-infrared Spectroscopy (NIRS) For a clinician, it is important to understand the basic principles of NIRS, to know the limits of use and to interpret them correctly. NIRS is a technique in...

01 January 1970 6,360 2 View

How to determine Vitamin D3 on paper based technique ?

Could you pls let me know how we can determin Vit D3 on paper base technique ? I mean how we can load the antibody on it and how that solution run to detect the vit D3 on band? Their are vitamin...

01 January 1970 451 5 View

Diagnostic Tests Accuracy: Systematic Review & Meta Analysis

Cochrane has provided guidance on how to perform systematic reviews & meta analysis of ”diagnostic tests accuracy” studies [SR & MA of DTA] (see link below). Are there software resources...

01 January 1970 238 4 View

What is updated methods in diagnosis of medical parasites in stool specimens ?

parasitic infection. Updated diagnostic methods

01 January 1970 2,388 4 View

What are the best ADHD diagnostic methods, given that my goal is to associate between risky driving and sub-impairments (or sub-dimensions) of ADHD?

Hi, I am studying the links between ADHD sub-dimensions and the different types of risky driving. What are the best ADHD diagnostic methods, given that my goal is to associate between risky...

01 January 1970 2,910 1 View

Hypothesis:   Non-invasive method to detect thyroid nodule malignancy: Near-infrared Spectroscopy (NIRS)

Near-infrared Spectroscopy (NIRS) For a clinician, it is important to understand the basic principles of NIRS, to know the limits of use and to interpret them correctly. NIRS is a technique in...

01 January 1970 5,282 2 View

What is updated methods in diagnosis of medical parasites in stool specimens ?

parasitic infection. Updated diagnostic methods

01 January 1970 1,789 4 View

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01 January 1970 8,543 0 View

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01 January 1970 9,548 0 View

What are the microbiological laboratory diagnostic tests to be performed to study Moraxella pneumoniae? | ResearchGate

I like to know what are the clinical diagnostic methods for Moraxella pneumonia?

01 January 1970 2,361 0 View

Can anyone suggest a commercial modified agglutination test (MAT) kit to detect Toxoplasma gondii antibodies in animals? | ResearchGate

I am looking forward to detect toxoplasma exposure in animals using Modified Agglutination Test (MAT), It seems the bioMérieux Toxoplasma MAT kit has discontinued its production. Can anyone...

01 January 1970 5,309 0 View