How many microsporidia spores are needed in stool for sufficient DNA extraction?

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Junaid Iqbal

Its simple simple my friend. Take any stool sample, which is PCR-negative for spores. Spike it with different number of spores, extract DNA and do PCR.

Yuri Tokarev

you never know - so the empirical approach suggested bt Junaid Iqbal sounds good. Try this assay several times to see the variation range.

Hamed Mirjalali

your advise is good, I tried that, but the results were not equal.I want to know other researchers experiences in this subject, if they had.