What is the best treatment to block autophagy in C2C12 cell line?

I read that NH4CL and chloroquine treatments can be used to block autophagic flux. Are there any other acceptable treatments?

02 September 2020 4,252 3 View

How to generate heterozygous knockouts using CRISPR Cas9 in iPSCs?

Hi all. I'm trying to create a heterozygous deletion (55bp) mutation in iPSCs to model malignant tranformation. I'm using two sgRNAs in a plasmid based system (puromycin selection). I obtained ~20...

18 May 2020 5,389 1 View

Are there guidelines for conducting a meta analysis and scoping review on case reports?

Currently, I can only find case reports on the rare condition I am planning to conduct a meta-analysis on. I will be evaluating outcomes and efficacy of treatments. Are there any known guidelines...

24 February 2020 5,742 3 View

5x5 crosstabs table on spss, iteration keeps going up and no results , anyone know why?

Hey all, So im trying to run 5X5 crosstabs with fisher exact test on SPSS, got about 1000~ lines in the file. Im seeing the iteration number keeps going up (1700 currently), but nothing happens...

07 December 2018 5,602 4 View

PVA freezing/thawing cycles - when is it better to "pause"?

I am currently working with PVA using the physical polymerization method, of multiple freezing/thawing (F/T) cycles. Since I've been getting opposite opinions regarding this question, I wanted to...

25 February 2017 2,797 1 View

How to oxidize Nitric oxide?

20 June 2016 2,008 9 View

Where can I purchase Silica nanoparticles?

In the past I worked with silica nanoparticles bought from "Nissan Chemicals", Product No. ST-40, see link for other types.In the company site I can't find that silica anymore.How can I get that...

17 August 2014 6,289 10 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View