How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

How to calculate the Atomic Polar Tensor using DFT ?

Dear friends, I am a beginner at DFT calculation. I have done a structure optimization using DFT- B3LYP/6-31G*, Nevertheless, I don't know How to calculate the Atomic Polar Tensor (APT) in...

17 February 2021 9,082 3 View

How to do mono formylation of N,N-diethyl-3-methoxybenzamide?

I have attempted the formylation of the above mentioned compound using a very well reported protocol using secondary Butyl Lithium and TMEDA at - 78 degrees Celsius. I have done it quite a few...

08 February 2021 2,793 2 View

Should I make 2 helical antennas? One for 2.4 GHz and another for 5.8 GHz?

Should I make 2 antennas, one for 2.4 GHz and one for 5.8 GHz? How should I approach the problem if I want to design the antennas which cover 400 and 900 MHz and 2.4 and 5.8 GHz? All together in...

26 January 2021 906 3 View

SPSS - PLUM: Shall I introduce binary variables as covariates?

I am doing an analysis of interaction effects, using PLUM in SPSS. My question is if the binary variables, that i have constructed to analyze the interaction effects, should be introduced as...

17 January 2021 8,217 4 View

Submission of the Materials Studio jobs to the remote Linux cluster?

I am doing some simulations using the Forcite module in Materials Studio. As the speed of this calculation on my workstation computer is unacceptably slow, I am trying to submit my job to the...

14 January 2021 8,653 1 View

Guidance in choosing a column for UHPLC quantification of organic acids in cranberry and blueberry fruit?

07 January 2021 6,214 3 View

How aromatics can dissolve asphaltene ?

As we all know asphaltene is defined as " The highest molecular weight component in the crude oil that is insoluble in light n-alkanes such as n-pentane or n-heptane and soluble in aromatics such...

05 January 2021 6,822 2 View

How can we apply svm for multiple classes?

for a dataset with 5 categorical classes , what would be the be the best classifiers ? size of data (670,49)

04 January 2021 5,187 6 View

Do you advice me how I can dissolve samples with different polarity in a culture media such as demem or emem??

We want to dissolve our research samples in cell culture media such as demem or emem. We want to conduct bio-assay guided technique to isolate bio-active compounds. But we have a difficulty to...

30 December 2020 3,590 3 View