Looking for high efficient light sources (Laser, HE LED) for Spectroscopic Ellipsometry?

Conventional light sources have a penetration depth of a few nanometers in metal layers and cannot be used for spectroscopic ellipsometry purpose to measure layer thicknesses of a few 100 nanometres.

27 October 2020 7,663 4 View

Why is fungal/bacterial DNA isolated with Qiagen Powerlyzer Powersoil Kit not stable?

We have isolated DNA with the Qiagen Powerlyzer Powersoil Kit (with addition of Proteinase K) for NGS downstream applications (16SrRNA/ITS2) and encountered the problem that the isolated DNA is...

26 October 2020 7,418 2 View

GHRH/GHRF antibody for rat brain immunohistochemistry?

I'm looking for an antibody (preferably validated) for growth hormone releasing hormone (GHRH/GHRF) for immunohistochemistry in rat brains. Do you have any leads? I would really appreciate your...

18 June 2018 5,384 1 View

How can I stain for dead neurons in the ischemic mouse brain?

I am looking for a staining method to label dead neurons in the ischemic mouse brain.I use coronar cryosection from mice which were subjected to transient MCAo and reperfusion. I tried NeuN+TUNEL...

09 June 2016 7,426 3 View

Is it possible to use desert sand as a building material?

Like mortar or concrete? I can not find case studies or articles about this topic?

15 May 2016 8,157 27 View

Is there recent knowledge about social structure within a litter of warthog piglets?

Young warthogs (Phacochoerus aethiopicus) display suspicious light colored bristels at their cheeks. In our view these seem to mimic the prominent tuskers of the adult...(?) I would greatly...

06 October 2015 1,983 4 View

Has anyone ever tried to detect Blue Jets, Elves and Sprites above Volcanic Eruption Clouds ?

Many years back, I attended a special session on Elves, Sprites and Blue Jets at AGU in San Francisco. These are typically observed above severe thunderstorm clouds and are observed at something...

27 August 2015 4,229 2 View

Is Qiagen AllPrep DNA/RNA FFPE kit compatible with Laser Capture Micro-dissection using arcturus capsure HS LCM caps?

08 March 2015 1,823 2 View

Is Indel Realignment recommended previous to Structural Variant Calling?

Is there any experience how GATK's IndelRealignment influences the results of subsequent Structural Variant Calling, e.g. with DELLY? Does Indel Realignment improve the predictions or should be...

27 November 2014 2,300 1 View

No title

01 January 1970 4,611 15 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Helix antenna coupling problem?

Dealing with a helix antenna, made with flexible material and not rigid like using a PCB board or wire. At the beginning of the helix is too close to the ground plane, causing coupling. How can I...

21 February 2021 4,453 3 View

Can bio-decomposer be a successful alternative to reduce residue burning?

To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...

16 February 2021 7,068 24 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Charmm/GROMACS Residue 'ZN' not found in residue topology database?

I am trying to do some MD simulations of a protein with zinc ions. I am trying to use a charmm forcefield (I have tried 2017 and 2021) and Gromacs. I get an error when doing pdb2gmx Residue 'ZN'...

12 February 2021 9,367 2 View

Is there any difference between "SEP" as a non standard residue and phohophorylated serine?

I am trying to run a molecular dynamics simulation for a ligand that contains a "SEP" residue. also, I am preparing my files using Charmm-GUI using charmm36 ff, but it says that it will rename my...

31 January 2021 6,321 1 View

How to calculate the error terms in 2-way mixed ANOVA?

30 January 2021 4,684 4 View

How to make a site-Selective Electroless Nickel Plating?

I would like to plating Ni/Au on the selected area. Can anyone give me a suggestion? It is a bit like to make golden finger on PCB board. Instead with the PCB board, I use polyimide as my...

28 January 2021 8,330 1 View

Why can I not find all of my residues in model predicted by Swiss-Model?

I am trying to perform homology modeling using the swiss-model but I have found that the swiss-model gives the same number of residues as that of the template. Whereas my sequence has more...

21 January 2021 5,568 3 View

Any resist stripper which can remove thick metal based polymer residue and which is compatible with doped silicon ?

I am looking some resist strippers which can easily remove metal based polymer residue after post dry etch process. I have some candidates below but not sure if they are compatible with doped...

21 January 2021 3,008 3 View