Hi all,
I am currently helping my colleague to do a drug screening assay. We have use dTag to "turn off" the protein expression of our protein of interest and treat the cell line with drugs combined with dTag/DMSO. I am just wondering if it is possible to see the synergistic effect with just on/off of the protein of interest. Some similar systems such as tet on/off would raise the same question. I am very intrigued if there is anything we can do with these systems that can just be turned on and off, but not adjusted to various level like normal drugs.
Many thanks!
The dTAG system is not strictly on/off, using different concentrations of the degrader compound allows you to modulate protein expression levels, which provides you with option to study drug impact in correlation to the levels of your protein of interest.
Thanks Sabine. I will keep that in mind.
I have used Bliss score for my experiment. I wasn't thinking straight and thought all synergistic scores are like ZIP. After I realised my misunderstanding I just went for Bliss. I'll just leave the answer here in case anyone ran into this same misunderstanding.
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