Need clarification about the capabilities of the Qubit, especially in distinguishing DNA purity from concentration.
Sumia Alam Good day! Based on my experience, the Qubit fluorometer provides approximate measurements, so it’s best to perform multiple replicates. If you notice significant variation between replicates, this could indicate the presence of impurities or inconsistencies in the sample.
No, un fluorómetro Qubit no mide directamente la pureza del ADN. Mide con precisión la cantidad de ADN, ARN y proteínas, pero no su pureza. La pureza se refiere a la ausencia de contaminantes, como proteínas o ARN, en una muestra de ADN.
Hi, to my knowledge, Qubit doesn't measure purity of DNA, but it measures accurate concentration of DNA. But because it measures specifically DNA and nothing else, it cannot tell you anything about the purity.
Tomáš Hluska Hello!
Yes, the Qubit fluorometer can measure DNA concentration using DNA-specific assays (a key point of interest for the author). However, it can also measure protein concentration if the corresponding Protein Assay Kit is used. By comparing results from both assays, one could estimate protein contamination in a DNA sample - though this requires separate measurements and is not a direct quantitative method for contamination.
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