Searching for a kit similar to this one from Abcam (ab109716) that is specified for mouse tissue homogenates.
Has anyone measured ATP synthase activity from homogenized mouse tissue before and can recommend another commercial kit?
Which variable data should be used for inflation in India? The CPI (Combined) is available only from 2011. And WPI, and GDP deflator is not a good indicator. I need quarterly data from...
30 May 2024 814 1 View
I am running a DFM model on STATA using timeseries data to derive the estimated Factor (through MLE) as the index. 1. Does the factor estimates derived from Dynamic Factor Model is zero for the...
30 May 2024 3,038 0 View
Hi I am new to STATA. I want to use the similar commands as this attached paper to create an index using DFM (Dynamic Factor Model) with panel data on...
06 May 2024 2,102 0 View
Can we perform moving average imputation for missing data in 2 or more stages? If yes, are there any conditions?
31 March 2024 1,040 1 View
I have to use PCA and DFM as two alternate methods for creating a banking stability index. 1. I want to know theory and application of DFM, what actually is it, where to use it and why, in easy to...
26 March 2024 1,491 1 View
Hello I have two questions: 1. Can I use more than one method for filling the missing values? Like moving averages, and imputing mean where moving averages didn't work? 2. Are the methods for...
21 March 2024 7,665 5 View
Hi everyone, Recently, I bought a new cell line named Tenocytes from a company. https://www.angioproteomie.com/ccp6601-human-tendon-cells-28tenocytes29-cap-0041.htm I followed the...
07 March 2024 6,149 3 View
Hi, I am phd student and I am working on plankton specie called Pseudo-nitzschia. I sent my first enviromental samples (filtered from sea-water) to Novogen for Ilumina sequencing and got some bar...
22 January 2024 4,923 2 View
As traditionally death and the process towards and after death has been the territory of spirituality and religion, its study is multifaceted and goes beyond purely biological and social issues....
29 November 2023 1,160 18 View
Hello everyone, I am working on system for calibration for the GPC/SEC system with multiple detectors and it requires that know parameters such as the , its concentration, dn/dc, extinction...
27 November 2023 4,989 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View