Dear all I wish to ask that meaning of Transmittance % (Example 20 %) at 200 nm wavelength in UV Spectrophotometer can be judged as that " 20 % Particles in our solution are at 200 nm". Is my approach correct or not. Please suggest...
You are not correct. 20% transmittance only means that the detector of that spectrometer only detects 20% of the photons that enter the sample. To know something about the particle size you need a rather different experiment, and it may even depend on the material that composes the particles.
Sorry to say but You are totally wrong .... % transmittance is the amount of light energy that passes through the sample. For example, there is a signal around which transmittance is 80% that means that 80% light passes (transmitted) through the sample and 20% of the light is absorbed respectively. Now comes to your question, 20% transmittance means that the sample absorbs 80% of the light and 20% is transmitted through the sample.
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