during spectrophotometer analyzing for a solution contains nanoparticles and protein I get 2 peaks at 420 and 280 nm . How can interpret these peaks ?
Dear Yuri Mirgorod
silver nanoparticles and fungal filtrate
with my regard
280 nm is band corresponds to the pi-pi* transition electrons in benzene ring of protein.
420 nm is plasmon bands of Ag nanoparticles. See our articles.
More specifically the 280 nm absorption is due to tryptophan and tyrosine.
http://omlc.org/spectra/PhotochemCAD/html/091.html
http://omlc.org/spectra/PhotochemCAD/html/092.html
Hi
https://chem.libretexts.org/Textbook_Maps/Organic_Chemistry_Textbook_Maps/Map%3A_Organic_Chemistry_(McMurry)/Chapter_14%3A_Conjugated_Compounds_and_Ultraviolet_Spectroscopy/14.08_Interpreting_Ultraviolet_Spectra%3A_The_Effect_of_Conjugation
Silver nanoparticles (AgNPs) gives an absorption peak in the range 400 nm to 480 nmٍ.Peak around 420nm belongs to AgNPs. Formation of nanoclusters or precursor you used may be caused a peak at 280 nm.
dear dr. see this linkhttps://www2.chemistry.msu.edu/faculty/reusch/.../spectrpy/uv-vis/spectrum.htm
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