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Questions related from Yugendran T
We are intending to purchase a Real-Time PCR. Which instrument performs better? Whether to purchase Peltier based model or rotor based model?
08 December 2019 5,003 4 View
I tried extracting DNA from Garcinia fruit and got the following readings with Nanodrop. Nucleic acid: 149.7 ng/µL A260/A280: 1.39 A260/A230: 0.12 A260: 2.99 A280: 2.15 Factor: 50 Baseline...
31 July 2019 5,557 9 View
Hi all, I tried extracting DNA from Garcinia dried fruit using R-Biopharm SureFood Prep Basic and Qiagen DNeasy Plant Mini Kit. I also tried extracting the DNA following the protocol described in...
31 July 2019 559 0 View
Hello everyone, I am carrying out TaqMan probe based Real-Time PCR assay. I am able to observe signal peak for the NTC sample. However upon agarose electrophoresis I was unable to see any band...
18 July 2019 6,752 6 View
The present aim of my study is to differentiate the cross breeds from the parent breeds. I know an insertion sequence which is present in one of the parent breed. Will this insertion sequence...
05 June 2019 9,961 4 View
We perform Sanger sequencing for varied range of PCR products. We usually employ ExoSAP for PCR product clean-up reaction. Due to unavoidable reasons we are looking for ExoSAP alternative. Does...
06 May 2019 928 4 View
I tried extracting DNA from an unknown environmental fungal isolate using Qiagen DNeasy extraction kit. However, I was unable to extract DNA in the first attempt. Then I tried increasing the...
26 March 2019 6,106 12 View
I extracted total RNA from plant tubers using Macherey-Nagel RNA extraction kit. Upon Nanodrop examination I got following results; Nucleic acid concentration: 15.5 ng/uL A260: 0.39 A280:...
25 February 2019 4,921 0 View
The commercially available bllod samples are mostly either defribinated or lysed. I need to procure the blood sample for genomic DNA extraction purpose. Is it possible to extract genomic DNA from...
08 February 2019 2,273 7 View
I have gone through few of the literature available over Pubmed and other online resources and could find kits that are capable of identifying BSE prions from brain tissue samples. Whereas, I am...
29 January 2019 7,797 3 View
I am looking for standardising a Taqman qPCR assay for detection purpose from various matrices. My query is whether, we can use degenerative probe sequences for the same? Do degenerative probe...
03 January 2019 6,612 0 View
In livestock farming slaughter age is important as meat sold from younger animals are often regarded as superior to the meat of older animals. Eg. - Veal is expensive compared to beef similarly,...
01 January 2019 982 10 View
Though females carry two X chromosome, only one chromosome gets expressed and remains active throughout the lifetime. In the backdrop of this information I just to know the pathways that govern...
05 November 2018 285 3 View
The aim of the analysis is to detect the presence of Cryptosporidium parvum oocysts and Giardia lamblia cysts in the given water sample. Majority of the literature available have employed Dyna...
26 October 2018 1,854 3 View
The way 16S and 18S rDNA sequencing employed for the genetic level identification of bacteria and fungi respectively, am looking for sequence based identification of the plant species targeting...
06 September 2018 5,116 3 View
When I dissolve 1g of CTAB separately it forms a clear solution, but when I add 1g of SDS to the CTAB solution, it forms a precipitate. I add 1g of SDS and CTAB to prepare the buffer. What are the...
31 March 2018 4,006 6 View
I have basic knowledge for nucleotide sequence analysis and gene annotation. I am looking forward for certification course which would give an insight into NGS technology, alignment of reads...
20 February 2018 4,044 3 View
I want to know the oxidation state of the iron ion present in the Muller-Hinton agar. Whether it is ferrous ion or ferric ion?
24 May 2016 9,205 2 View
I have sequence with a novel mutation at a particular nucleotide position within the gene encoding sequence. How can I submit this particular sequence to GenBank and obtain the accession number?
03 May 2016 1,854 5 View
i am looking for quantifying the transcript level of certain gene in the presence and absence of the drug pressure. The Ct value of my target gene is comparatively higher in the presence of the...
18 March 2016 2,563 8 View
I am studying a new SNP present in bacterial antibiotic resistance gene. qPCR based genotyping for this SNP has not been carried out so far. I designed a Taqman chemistry based SNP genotyping...
03 July 2015 5,355 4 View
I intend to carry out relative gene expression analysis for genes conferring antibiotic resistance using Taqman based qPCR assay. I want to quantify the mRNA level from E. coli and K. pneumoniae...
30 April 2015 881 3 View
How to separate an enzyme from its cofactor?
20 March 2015 7,486 4 View
I need to receive bacterial control strains from a lab in the USA. So what are the MTA guidelines for doing this?
19 November 2014 3,901 1 View
