Yugendran T

14 Questions 22 Answers 0 Followers

Questions related from Yugendran T

Which type of qPCR machine performs better; Peletier based or rotor based?

We are intending to purchase a Real-Time PCR. Which instrument performs better? Whether to purchase Peltier based model or rotor based model?

08 December 2019 4,926 4 View

Discrepency between nanodrop reading and agarose gel electrophoresis interpretation?

I tried extracting DNA from Garcinia fruit and got the following readings with Nanodrop. Nucleic acid: 149.7 ng/µL A260/A280: 1.39 A260/A230: 0.12 A260: 2.99 A280: 2.15 Factor: 50 Baseline...

31 July 2019 5,460 9 View

What is the efficient method for extracting DNA from Garcinia dried fruit?

Hi all, I tried extracting DNA from Garcinia dried fruit using R-Biopharm SureFood Prep Basic and Qiagen DNeasy Plant Mini Kit. I also tried extracting the DNA following the protocol described in...

31 July 2019 502 0 View

What can be the possible reason for the signal observed in NTC during TaqMan probe based qPCR (Real Time PCR) assay?

Hello everyone, I am carrying out TaqMan probe based Real-Time PCR assay. I am able to observe signal peak for the NTC sample. However upon agarose electrophoresis I was unable to see any band...

18 July 2019 6,698 6 View

Why fungal DNA does not yield amplification against ITS-1 and ITS-4 primers?

I tried extracting DNA from an unknown environmental fungal isolate using Qiagen DNeasy extraction kit. However, I was unable to extract DNA in the first attempt. Then I tried increasing the...

26 March 2019 6,005 12 View

How the X chromosome expression regulated among carrier females?

Though females carry two X chromosome, only one chromosome gets expressed and remains active throughout the lifetime. In the backdrop of this information I just to know the pathways that govern...

05 November 2018 181 3 View

Is universal primers specific for rbcL gene in plants available?

The way 16S and 18S rDNA sequencing employed for the genetic level identification of bacteria and fungi respectively, am looking for sequence based identification of the plant species targeting...

06 September 2018 5,038 3 View

How to avoid precipitation while making CTAB - SDS buffer?

When I dissolve 1g of CTAB separately it forms a clear solution, but when I add 1g of SDS to the CTAB solution, it forms a precipitate. I add 1g of SDS and CTAB to prepare the buffer. What are the...

31 March 2018 3,922 6 View

Can anyone suggest a constructive certification course for "Next generation sequencing"?

I have basic knowledge for nucleotide sequence analysis and gene annotation. I am looking forward for certification course which would give an insight into NGS technology, alignment of reads...

20 February 2018 3,977 3 View

Muller-Hinton agar contains ferrous or ferric ion?

I want to know the oxidation state of the iron ion present in the Muller-Hinton agar. Whether it is ferrous ion or ferric ion?

24 May 2016 9,143 2 View

How upload a sequence with novel mutation in GenBank?

I have sequence with a novel mutation at a particular nucleotide position within the gene encoding sequence. How can I submit this particular sequence to GenBank and obtain the accession number?

03 May 2016 1,790 5 View

Which gene is best suited to serve as reference gene / internal control gene for relative gene expression studies in E. coli and K. pneumoniae?

I intend to carry out relative gene expression analysis for genes conferring antibiotic resistance using Taqman based qPCR assay. I want to quantify the mRNA level from E. coli and K. pneumoniae...

30 April 2015 799 3 View

What are cost effective procedures to dissociate metal ions from enzymes?

How to separate an enzyme from its cofactor?

20 March 2015 7,441 4 View

What are the MTA guidelines for receiving bacterial strains from abroad in India?

I need to receive bacterial control strains from a lab in the USA. So what are the MTA guidelines for doing this?

19 November 2014 3,832 1 View