The present aim of my study is to differentiate the cross breeds from the parent breeds. I know an insertion sequence which is present in one of the parent breed. Will this insertion sequence appear within the gene sequences of the cross breed progeny?
If that insertion is homozygous in that one parent, each of the progenies will show that insertion in a heterozygous condition.
If that insertion is heterozygous for that parent, 50% of the progeny will be heterozygous as well, the other 50% would be homozygous as non-insertion carrierer.
So if you find one genotype that is homozygous for the insertion and one that is homozygous for non-insertion and all others are heterozygous, both homozygous are the parents and the heterozygous are the progeny. Otherwise you cannot distinguish them for sure.
Stephan Schie Thanks for your answer. It was really helpful. Is there any method to detect the heterozygous condition for this insertion sequence?
Do you have the possibility to make a molecular marker for that region ?
For example on agarose gel.
How great is that insertion in basepairs ?
The insertion sequence is 16bp long.
I have no idea regarding the preparation of customised molecular marker. I employ 100 bp molecular marker.
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