The commercially available bllod samples are mostly either defribinated or lysed. I need to procure the blood sample for genomic DNA extraction purpose. Is it possible to extract genomic DNA from defibrinated blood?
In defibrinated blood, the fibrin is mechanically removed during the clotting process without the presence of anticoagulants or other additives. However, the DNA is still present. The procedure for the DNA extraction is the same as in the extraction of DNA from blood as shown in the link below.
It is advisable to process defibrinated blood on time as it is easy to degrades due to the presence of some autolytic enzymes (DNAase) that can destroy the DNA.
Article Methods for extracting genomic DNA from whole blood samples:...
Yes! Very possible as only fibrinogen (a protein) has been removed but the blood cells containing the DNA are still intact, at least for some time.
The procedure for the DNA extraction is the same as in the extraction of DNA from blood
Tombari Pius Monsi Maurice Ekpenyong Basil A. Abbas Thanks for clarifying my doubt. Your explanation was really helpful.
Maurice Ekpenyong , please, is it possible to still recover DNA from defibrinated horse blood stored for over 3 months at 2-8 degrees celcius?
@Mark Ezegbogu
Yes, it is but you shouldn't expect a good quality extraction because that time mentioned by you is maximum time that scientist consider for stability of DNA, in best of my knowledge.
Regards
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