I am studying a new SNP present in bacterial antibiotic resistance gene. qPCR based genotyping for this SNP has not been carried out so far. I designed a Taqman chemistry based SNP genotyping assay for identifying this SNP.
But, recently a group has published their work on qPCR based genotyping of the same SNP. They have developed an assay for genotyping the wildtype and mutant alleles based on high-resolution melting data using unlabelled probes.
Should I still proceed with my study. Does Taqman chemistry based genotyping method have any advantage over HRM method? Will the assay which I have designed be still novel?
A taqman assay should be very robust, it's difficult to go wrong with one. I'm not sure why your assay needs to be novel, your results are what you are interested in.
Both assays are reliable. What do you mean about "novelty"? The principle, of cause, is not novel. However, if one designs a diagnostic assay for a SNP for which these assays have not been applied previously, this would make an original piece of work. As for me, this is OK for a bachelor or master thesis and possibly publishable if put in a right context :)
Dear Andriy Khobta,
Thanks for your answer.
I am a PhD student from Dept. of Microbiology, JIPMER, India. I have designed a diagnostic assay for this SNP using Taqman chemistry which,has not been applied for the same till now. Hence, I considered it as an original piece of work. But, last month diagnostic assay for same SNP has been published based on HRM. An article being published already will the assay I have designed hold good.
Lookig forward to your reply.
Dear Christopher,
Thanks for your message. SInce, there was no previous publication regarding the diagnosis of the SNP using qPCR I thought my work would be novel and have certain impact. But, an artcile already being published I was thoughtful about my assay.
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