How effectively extract DNA from quartz filter and liquid?

Good evening, I am working on the establishment of DNA extraction from quartz filter and collection liquid that were used for air sampling. The idea is to extract as much as possible DNA of high...

03 February 2020 4,323 2 View

Difference between Polycarbonate and quartz filter?

Dear colleagues, I have just started to work on the environmental project. I have to analyze the microorganisms in the air. For that I have to choose the correct type of filters. In my group, 2...

17 June 2018 4,697 3 View

How to identify fungi?

Hi all, I am searching for kits (apart from MicroSeq, PCR) or other simple techniques to identify at least the class of fungi. Any ideas? Thank you in advance, Sofya

03 June 2018 1,143 9 View

How do I do live immunostaining and sorting of cryopreserved monocytes?

i would like to do surface immunostaining and live sorting of cryopreserved CD14+ human monocytes for further experiments. Does anybody have experience with this kind of experiments? Do I need to...

12 February 2016 1,782 5 View

How can I improve transfection in H9C2 cells?

17 May 2015 4,583 6 View

Does someone see a difference in the morphology of H9C2 cells during culturing?

I am working with H9C2 cell line and I see difference in morphology of this cells during the culturing. They can be round as well as prolonged ones and it seems to me that the round shape appears...

14 December 2014 7,098 4 View

With H9C2 how can I check the differentiation of the cell line?

H9C2 cell line can differentiate in cardiac or skeletal cells. I have to show that my cells are definitely cardiac ones. Does anyone have experience with this?

08 April 2014 5,420 4 View

CGMP ELISA based assays

I am trying to measure cGMP in H9C2 cells. The data I get are out of the calibration curve. Does anyone has an experience or idea how this could be improved? I already increased cell number and it...

01 January 1970 7,121 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Which is better medicine or food in obesity?

Preference in terms of the scientific aspect

28 February 2021 796 2 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View