40 Questions 19 Answers 0 Followers
Questions related from Shilpi - Gupta
Hi, anyone have suggestions for crispr cleavage detection using thermo genomic cleavage detection kit? I am trying to make nfkb1 knock out in RAW 264.7 cell line and I have using thermo Cas9...
12 December 2018 4,211 0 View
hi, anyone can suggest some lab in us working on crisp cell line knock outs?
09 September 2018 8,124 0 View
Hi, all.I need to know about gene and protein name nomenclature? Basically as per rules we need to write protein name in capital letters and gene name first letter capitals and rest in small...
09 September 2018 305 0 View
Hi all, I need to can we measure i-NOS in h.pylori treated samples collected in PBS? After infection of any cell line , we usually washed cells with PBS before collection of cell pellet for RNA...
05 May 2018 3,983 2 View
Hi, anyone has information regarding peoples working on crispr- cas9 in mouse cell lines? I want to apply for GET-in fellowship, so I need to contact person's working on same platform in which I...
05 May 2018 247 1 View
Can anyone has standardized protocol for Transfection in raw cell line? I have Thermo Ltx reagent? Is it vary with plasmid type? I have Pcdna 3with flag and his tag?? please suggest plasmid conc....
01 January 2018 1,236 5 View
HI, all I am working on H.pylori strain 26695 since 2 years but now its showing nay increase in il-beta or Inos production when infecting raw cells with same. this is bacteria we can borrowed from...
01 January 2018 5,638 1 View
Hi, I need to know about cell counting for time point experiments- I am working on Raw 264.7 cells which has 11 hours doubling time. I use to seed 1 Million cells in six well plate which has...
01 January 2018 669 3 View
Hi, All Can anyone tell me is JNU has license to import knock out mice?
12 December 2017 566 0 View
Hi, All, I like to know can anyone tell me or suggest me some labs in India preferably Delhi, who have done knock out in mammalaina cells using crisper technology.
10 October 2017 8,679 0 View
Dear All, is anyone has idea for international conference in 2017 on Helicobacter parthenogenesis, host immune response etc.
07 July 2017 5,821 2 View
HI, all, I am making heat map using MEV but its not giving good resolution and we have possibility of saving only in four formats, as listed above. For changing resolution using illustrator I need...
05 May 2017 8,971 0 View
Hi, I have Luminex bead cytokine assay data for mice, I need to represent them by web graph,do anyone have idea for the same .I have four conditions??
05 May 2017 5,357 0 View
HI all, I need to know formation of various Homo and hetero dimers of nfkb at control and infection condition in Raw cell line, so is serial immuno-precipitation is one of the choice?? basically i...
03 March 2017 9,152 0 View
Hi all, I need to do co-transfection in RAW cells by pcDNA3 in which I have neomycin selection, I already have my gene of interest with flag tag and one with his tag in the same vector. I have...
01 January 2017 7,158 2 View
HI all, Has anyone idea for protease inhibitor for Raw cells? I am making cytoplamsic and nuclear fraction using PIERCE Niper cytoplamsic and nuclear kit. I am making fractions at four different...
12 December 2016 8,156 3 View
hi, anyone can give suggestion for fractions preparation with pierce NEPER kit, I am getting contamination in my nuclear fraction when I am probing with GAPDH. I try to troubleshoot and...
10 October 2016 5,859 2 View
HI, I am working on nfkb1 and I need to check its expression on Raw and THP-1 cells by western as well I need to immunoprecipitation for its interacting partners. I have taken p50 ab from...
09 September 2016 3,804 3 View
hi, anyone please suggest me technique to detect presence of homodimer formation. I need to know homodimer formation of mouse nfkb1 which has mol wt of 50 Kda and when i am infecting raw cells...
09 September 2016 6,325 3 View
Hi, anyone has idea which GO database provides percent involvement of differentially regulated genes in particular pathways (biological, molecular and cell component) apart from Panther-go analysis
09 September 2016 9,656 2 View
Hi All, I need to perform double transfection in raw 264.7 with mouse p50-flag tag and his tag to know p50:p50 homodimer formation? I need to know is position of tag going to affect...
09 September 2016 3,166 1 View
I have mouse p50- c-terminal flag tag I PCDNA vector but I need two tags one with his as well, so it is important that his tag is also on C-terminal ad pcdna 3 only?? can I use pcdna3.1??I need to...
08 August 2016 3,058 1 View
Hi all, could anyone has idea what could be the reason for getting cytoplasmic contamination in nuclear fractions. I am making fractions of Raw as well Thp-1 cells but I am getting bands with...
08 August 2016 4,891 4 View
hi all, anyone had idea that any bacterial infection particularly H.pylori on cell lines(innate immune cell lines like-THP-1, Raw264.7, U937) or any other cal lead to complete repression and...
07 July 2016 2,747 1 View
Hello I'm looking for transcription factor resource that would give a list of transcription factors and their corresponding target gene. The species I'm mostly interested in are Mouse.
07 July 2016 6,991 1 View
can anyone suggest commercially available p50 *nfkb1)knock out any macrophage cell line particularly Raw264.7.
06 June 2016 7,189 1 View
hi, could anyone tell me database for pro-inflammatory and anti-inflammatory cytokines.
06 June 2016 764 5 View
hi, I need to know whats the diff between individual ip and western with that particular ab? If I need to check expression of one subunit of large five member complex like in my study p50 of nfkb...
05 May 2016 4,551 3 View
What is a suitable nuclear/ cytoplasmic loading control to use for westerns on nuclear lysates made from raw cells for detecting nfkb activation and its subunit expression?
05 May 2016 5,444 3 View
HI all, can anyone tells me if we want to infect any cell line with bacteria so can we seed cells up to maximum amount we can seed in an dish or less? like in six well i use to seed 1 million o/n...
05 May 2016 8,738 2 View
Does anyone has luck with -NE-PER Nuclear and Extraction Reagents for raw 264.7 raw and thp-1. or else anyone can suggest some other brand kit for same cell lines.
05 May 2016 5,345 1 View
can anyone has standard set protocol for NFkb?? in raw cells and thp-1
05 May 2016 2,752 2 View
i need to whats the exact difference as these are very related term and suppose i need to study effect of H.pylori(bacteria) on innate immune cell line such as -U937, thp-1 and raw etc, how we can...
05 May 2016 3,104 3 View
I need to know how exactly H.pylori activates NF-kb?? is it strain specific? is it vary from cell lent o cell line.
05 May 2016 5,830 3 View
I need to know hoe we can calculate how much induction of inos is there in treated compared to control with absorbance value. Hoe to use standard curve value in exp values??
05 May 2016 7,232 4 View
Hi, I need to know if 26695 produces inos? I am treating raw 264.7 cells with H. pylori strain and I need to know wheter infection is there, since there is upregulation of il-beta in treated...
05 May 2016 8,897 3 View
anyone has suggestion for transfection with raw 264.5? which reagent either lipofection or nucleofection will works? is it necessary to use same vector for two different tags for same gene for...
05 May 2016 5,427 6 View
hi, could anyone has his tag and myc tag of nfkb1(p50) or else someone can suggest me name of labs or peoples in your circle if you know someone.
05 May 2016 955 2 View
could anyone tell me if we standarize primer concentration(0.1-0.5uM), then which we should use for further qpCR analysis if we are getting no primer dimer in either case, ideally one should take...
04 April 2016 2,130 3 View
HI, ANYONE KNOW HOW TO DO SPECIFIC GO ANALYSIS WITH INFLAMMATION OR IMMUNITY RELATED GENES, GENERALLY GO CLASSIFY GENES ON THREE BASIS-MOLECULAR FUNCTION, CELL COMPONENTS AND BIOLOGICAL PATHWAYS.
04 April 2016 988 1 View
