Hi, All
Can anyone tell me is JNU has license to import knock out mice?
Hi, anyone have suggestions for crispr cleavage detection using thermo genomic cleavage detection kit? I am trying to make nfkb1 knock out in RAW 264.7 cell line and I have using thermo Cas9...
11 December 2018 4,167 0 View
hi, anyone can suggest some lab in us working on crisp cell line knock outs?
08 September 2018 8,080 0 View
Hi, all.I need to know about gene and protein name nomenclature? Basically as per rules we need to write protein name in capital letters and gene name first letter capitals and rest in small...
08 September 2018 254 0 View
Hi, anyone has information regarding peoples working on crispr- cas9 in mouse cell lines? I want to apply for GET-in fellowship, so I need to contact person's working on same platform in which I...
04 May 2018 194 1 View
Hi all, I need to can we measure i-NOS in h.pylori treated samples collected in PBS? After infection of any cell line , we usually washed cells with PBS before collection of cell pellet for RNA...
04 May 2018 3,931 2 View
Hi, I need to know about cell counting for time point experiments- I am working on Raw 264.7 cells which has 11 hours doubling time. I use to seed 1 Million cells in six well plate which has...
31 December 2017 618 3 View
HI, all I am working on H.pylori strain 26695 since 2 years but now its showing nay increase in il-beta or Inos production when infecting raw cells with same. this is bacteria we can borrowed from...
31 December 2017 5,592 1 View
Can anyone has standardized protocol for Transfection in raw cell line? I have Thermo Ltx reagent? Is it vary with plasmid type? I have Pcdna 3with flag and his tag?? please suggest plasmid conc....
31 December 2017 1,194 5 View
Hi, All, I like to know can anyone tell me or suggest me some labs in India preferably Delhi, who have done knock out in mammalaina cells using crisper technology.
09 October 2017 8,631 0 View
Dear All, is anyone has idea for international conference in 2017 on Helicobacter parthenogenesis, host immune response etc.
06 July 2017 5,761 2 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I submitted my e-mail address on modeller website to get a license. The problem is that, the site cannot process my school e-mail (ewhain.net). I submitted my Lab website and school name so that...
25 February 2021 9,054 3 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View
