H.pylori activation of Nf-Kb signaling pathways?

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Crispr-cas9 knock out with Mouse cell line?

Hi, anyone have suggestions for crispr cleavage detection using thermo genomic cleavage detection kit? I am trying to make nfkb1 knock out in RAW 264.7 cell line and I have using thermo Cas9...

11 December 2018 4,235 0 View

Get-in fellowship?

hi, anyone can suggest some lab in us working on crisp cell line knock outs?

08 September 2018 8,147 0 View

Gene and protein name nomenclature?

Hi, all.I need to know about gene and protein name nomenclature? Basically as per rules we need to write protein name in capital letters and gene name first letter capitals and rest in small...

08 September 2018 330 0 View

Crispr-cas9 knock out with Mouse cell line?

Hi, anyone has information regarding peoples working on crispr- cas9 in mouse cell lines? I want to apply for GET-in fellowship, so I need to contact person's working on same platform in which I...

04 May 2018 269 1 View

Inos detection after H.pylori treatment in RAW 264.7 macrophages?

Hi all, I need to can we measure i-NOS in h.pylori treated samples collected in PBS? After infection of any cell line , we usually washed cells with PBS before collection of cell pellet for RNA...

04 May 2018 4,005 2 View

Cell counting for time kinetics Experiment?

Hi, I need to know about cell counting for time point experiments- I am working on Raw 264.7 cells which has 11 hours doubling time. I use to seed 1 Million cells in six well plate which has...

31 December 2017 697 3 View

H.pylori 26695 wild type strain?

HI, all I am working on H.pylori strain 26695 since 2 years but now its showing nay increase in il-beta or Inos production when infecting raw cells with same. this is bacteria we can borrowed from...

31 December 2017 5,660 1 View

Transfection protocol for raw cells?

Can anyone has standardized protocol for Transfection in raw cell line? I have Thermo Ltx reagent? Is it vary with plasmid type? I have Pcdna 3with flag and his tag?? please suggest plasmid conc....

31 December 2017 1,267 5 View

License for knock out permit?

Hi, All Can anyone tell me is JNU has license to import knock out mice?

11 December 2017 593 0 View

Preparation of cell line knock out using crisper?

Hi, All, I like to know can anyone tell me or suggest me some labs in India preferably Delhi, who have done knock out in mammalaina cells using crisper technology.

09 October 2017 8,702 0 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

Validated HPLC method for the estimation of the Amphotericin B ?

During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...

08 July 2024 1,330 0 View

Do you have a protocol for stimulation of mouse B cells by PWM?

Looking for a protocol for the stimulation of B cells with pokeween mitogen

22 June 2024 4,882 4 View

Asking for a vector, containing Hygromycin B resistance gene?

In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...

19 June 2024 7,822 3 View

LPS dose for RAW 264.7 NF-kB macrophage?

I am working on a project that requires stimulating RAW 264.7 NF-kB macrophage and readout my signal by luciferase assay. Although all concentrations tested (10, 50, 100ng/mL) after 24 hours...

16 June 2024 2,885 3 View

What are those vacuolated structures in my tilapia intestinal cross-sections (H&E stained)? Do the sections exhibit signs of enteritis?

Could someone please confirm if those vacuolated structures are goblet cells? And whether the sections exhibit signs of inflammation. Context: I am conducting histological analysis of the...

15 June 2024 3,208 4 View

Is there anything called cd 12 in immunology?

define cd

06 June 2024 9,533 5 View