HI, ANYONE KNOW HOW TO DO SPECIFIC GO ANALYSIS WITH INFLAMMATION OR IMMUNITY RELATED GENES, GENERALLY GO CLASSIFY GENES ON THREE BASIS-MOLECULAR FUNCTION, CELL COMPONENTS AND BIOLOGICAL PATHWAYS.
https://www.biostars.org/p/64791/
Hi, anyone have suggestions for crispr cleavage detection using thermo genomic cleavage detection kit? I am trying to make nfkb1 knock out in RAW 264.7 cell line and I have using thermo Cas9...
11 December 2018 4,235 0 View
hi, anyone can suggest some lab in us working on crisp cell line knock outs?
08 September 2018 8,147 0 View
Hi, all.I need to know about gene and protein name nomenclature? Basically as per rules we need to write protein name in capital letters and gene name first letter capitals and rest in small...
08 September 2018 330 0 View
Hi, anyone has information regarding peoples working on crispr- cas9 in mouse cell lines? I want to apply for GET-in fellowship, so I need to contact person's working on same platform in which I...
04 May 2018 269 1 View
Hi all, I need to can we measure i-NOS in h.pylori treated samples collected in PBS? After infection of any cell line , we usually washed cells with PBS before collection of cell pellet for RNA...
04 May 2018 4,005 2 View
Hi, I need to know about cell counting for time point experiments- I am working on Raw 264.7 cells which has 11 hours doubling time. I use to seed 1 Million cells in six well plate which has...
31 December 2017 697 3 View
HI, all I am working on H.pylori strain 26695 since 2 years but now its showing nay increase in il-beta or Inos production when infecting raw cells with same. this is bacteria we can borrowed from...
31 December 2017 5,660 1 View
Can anyone has standardized protocol for Transfection in raw cell line? I have Thermo Ltx reagent? Is it vary with plasmid type? I have Pcdna 3with flag and his tag?? please suggest plasmid conc....
31 December 2017 1,267 5 View
Hi, All Can anyone tell me is JNU has license to import knock out mice?
11 December 2017 593 0 View
Hi, All, I like to know can anyone tell me or suggest me some labs in India preferably Delhi, who have done knock out in mammalaina cells using crisper technology.
09 October 2017 8,702 0 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View